Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604212036_d4tz GAAACTGGAAGGACCCCACGCACCCTCCCAGG(+ACTCAC)GTACCGCCCAGCCCTCTCATGTGTGATTCTG NGG 10 8 17 8 24 0 100 5 10
    240604212034_8i5d AAATTCAGAATCACACATGAGAGGGCTGGGCG(+ACTCAC)GTACCCTGGGAGGGTGCGTGGGGTCCTTCCA NGG 10 8 17 8 24 0 100 5 10
    240604212031_p1nk GGTCTTCTGTTGACTTTGTCCTTTCCCCCCAG(+ACTCAC)GTACCGAGTTACTCAGTCTGCTCTCCACTGT NGG 10 8 17 8 24 0 100 5 10
    240604212030_re8h CATGGACAGTGGAGAGCAGACTGAGTAACTCG(+ACTCAC)GTACCTGGGGGGAAAGGACAAAGTCAACAGA NGG 10 8 17 8 24 0 100 5 10
    240604212028_vq5w GTCAGCCGCGTGTTCGAGCGGTGTCACAGCCG(+ACTCAC)GATCCTGCCCAGACTGCTGCCTGTGTGCTTT NGG 10 8 17 8 24 0 100 5 10
    240604212026_2vlj AAGGGAAAGCACACAGGCAGCAGTCTGGGCAG(+ACTCAC)GATCCGGCTGTGACACCGCTCGAACACGCGG NGG 10 8 17 8 24 0 100 5 10
    240604212024_hxvw GGACTGATCCGAAGACTCTATGCTGGAACCTG(+ACTCAC)GATCCTCATCATCTATGTATATAAGATCTTT NGG 10 8 17 8 24 0 100 5 10
    240604212023_xv1o TGCCAAAAGATCTTATATACATAGATGATGAG(+ACTCAC)GATCCAGGTTCCAGCATAGAGTCTTCGGATC NGG 10 8 17 8 24 0 100 5 10
    240604212021_5epp CTGTAAGACGTTTGCTTTGTAACTTGGTCACG(+ACTCAC)GATCCAAGCCCATGCTGGGGGAAATGGCACT NGG 10 8 17 8 24 0 100 5 10
    240604212020_ajjw GCACTAGTGCCATTTCCCCCAGCATGGGCTTG(+ACTCAC)GATCCGTGACCAAGTTACAAAGCAAACGTCT NGG 10 8 17 8 24 0 100 5 10