Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604212110_2rra CTATCTTCAGACACAACCAGAGGAGGGCATTG(+ACTCAC)GATCCTATTACCAATGGTTGTAAGCTACCAT NGG 10 8 17 8 24 0 100 5 10
    240604212109_8c5j ACCACATGGTAGCTTACAACCATTGGTAATAG(+ACTCAC)GATCCAATGCCCTCCTCTGGTTGTGTCTGAA NGG 10 8 17 8 24 0 100 5 10
    240604212107_f47c CCCACTCCCACTCCCACTCCCAACCCCCATTG(+ACTCAC)GATCCCTGGATTGTCAGTCAAGAATGAAAAA NGG 10 8 17 8 24 0 100 5 10
    240604212105_pwvv TGGGGTTTTTCATTCTTGACTGACAATCCAGG(+ACTCAC)GATCCAATGGGGGTTGGGAGTGGGAGTGGGA NGG 10 8 17 8 24 0 100 5 10
    240604212103_5xlv CCATTCATAGTTCCATAACTCCGGTTCTGTAG(+ACTCAC)GATCCGAGGTTCTTTTCTCACCCGTACGAAA NGG 10 8 17 8 24 0 100 5 10
    240604212101_avew ATGCATTTCGTACGGGTGAGAAAAGAACCTCG(+ACTCAC)GATCCTACAGAACCGGAGTTATGGAACTATG NGG 10 8 17 8 24 0 100 5 10
    240604212059_9f58 TCCTCAGAAAATTGGACATAGTACTACCGGAG(+ACTCAC)GATCCAGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240604212058_cmku TTCTGGATATATGCCCAGGAGAGGTATTGCTG(+ACTCAC)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240604212055_r2qv GGATGGACCATGTAGAGACTGCCATATCCAGG(+ACTCAC)GATCCACCCCATAATCAGCATCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240604212053_taim GGTGTCAGCGTTTGGATGCTGATTATGGGGTG(+ACTCAC)GATCCCTGGATATGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10