Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604212051_qjm5 CTGTGCTGCCAGCCCCTGCCCAGGTTCCTTTG(+ACTCAC)GATCCGCAGATGAAAGACACACACACACACA NGG 10 8 17 8 24 0 100 5 10
    240604212050_ktsb GTGTGTGTGTGTGTGTGTGTCTTTCATCTGCG(+ACTCAC)GATCCAAAGGAACCTGGGCAGGGGCTGGCAG NGG 10 8 17 8 24 0 100 5 10
    240604212049_zjuk TTAATTTCACAGATTCTTGTTCCAATGTCTAG(+ACTCAC)GATCCATACATGATCTTTAGGGCTCCTTCAA NGG 10 8 17 8 24 0 100 5 10
    240604212047_wpb9 GCCCTTTGAAGGAGCCCTAAAGATCATGTATG(+ACTCAC)GATCCTAGACATTGGAACAAGAATCTGTGAA NGG 10 8 17 8 24 0 100 5 10
    240604212046_0ji4 TTTAGATGATAAATTTCTCATGTCAATTTTTG(+ACTCAC)GTACCATTATTATCTATATTTAATAGATAAG NGG 10 8 17 8 24 0 100 5 10
    240604212045_861p TTTCTCTTATCTATTAAATATAGATAATAATG(+ACTCAC)GTACCAAAAATTGACATGAGAAATTTATCAT NGG 10 8 17 8 24 0 100 5 10
    240604212044_2nqe CTAAATTTGTAAATGGCAGCAGTATTATAAAG(+ACTCAC)GATCCAAATGACCTAACAGGAACCAGAAAAA NGG 10 8 17 8 24 0 100 5 10
    240604212042_prw2 CAATTTTTTTCTGGTTCCTGTTAGGTCATTTG(+ACTCAC)GATCCTTTATAATACTGCTGCCATTTACAAA NGG 10 8 17 8 24 0 100 5 10
    240604212041_2lhg AGACTCATGGTTCACCAAGTTGGACTTCGGTG(+ACTCAC)GTACCTGTCTTTTGGTCTGTTGTGGGCTCCC NGG 10 8 17 8 24 0 100 5 10
    240604212039_mso1 AGAGAGGGAGCCCACAACAGACCAAAAGACAG(+ACTCAC)GTACCACCGAAGTCCAACTTGGTGAACCATG NGG 10 8 17 8 24 0 100 5 10