Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604212145_kkme AGTGTATCTTCAAGAGACAAGAACACTTGCTG(+ACTCAC)GATCCGTTCACAAGGGTGAGGTTTAGCAACT NGG 10 8 17 8 24 0 100 5 10
    240604212144_6jif CTCTCAGTTGCTAAACCTCACCCTTGTGAACG(+ACTCAC)GATCCAGCAAGTGTTCTTGTCTCTTGAAGAT NGG 10 8 17 8 24 0 100 5 10
    240604212142_0kuv GAAGCATTAGATCCTGTCCTGAAGGAGTCTGG(+ACTCAC)GTACCCACTTAGTGTCCTCTGGTCAAAATGT NGG 10 8 17 8 24 0 100 5 10
    240604212140_v9np TAGAAACATTTTGACCAGAGGACACTAAGTGG(+ACTCAC)GTACCCAGACTCCTTCAGGACAGGATCTAAT NGG 10 8 17 8 24 0 100 5 10
    240604212139_q0tl GTTAACGTCCCCTGTACTTGGTATTAAGTAAG(+ACTCAC)GTACCGACGCTGAGCGAGCTCACGCTGCAGA NGG 10 8 17 8 24 0 100 5 10
    240604212137_ieho CCTAATCTGCAGCGTGAGCTCGCTCAGCGTCG(+ACTCAC)GTACCTTACTTAATACCAAGTACAGGGGACG NGG 10 8 17 8 24 0 100 5 10
    240604212135_nwn2 GGGTTTCTTCCTAGAATCTCAGCCCATGGGGG(+ACTCAC)GTACCACAGCAGGAAGATCATGAGTTTAGAG NGG 10 8 17 8 24 0 100 5 10
    240604212132_dy4w TATGGCTCTAAACTCATGATCTTCCTGCTGTG(+ACTCAC)GTACCCCCCATGGGCTGAGATTCTAGGAAGA NGG 10 8 17 8 24 0 100 5 10
    240604212131_x92g GAAGCATTAGATCCTGTCCTGAAGGAGTCTGG(+ACTCAC)GTACCCACTTAGTGTCCTCTGGTCAAAATGT NGG 10 8 17 8 24 0 100 5 10
    240604212129_mbks TAGAAACATTTTGACCAGAGGACACTAAGTGG(+ACTCAC)GTACCCAGACTCCTTCAGGACAGGATCTAAT NGG 10 8 17 8 24 0 100 5 10