Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604205025_unit GTCCTATTGTCATGTCAAAGGGTTGGACAAAG(+ACTCAC)GATCCTCTGCACATTTTGAACACTGTCAAAA NGG 10 8 17 8 24 0 100 5 10
    240604205024_4uf4 ATGAGCCCCGAGGGTCTGCCTGCGACTCGCAG(+ACTCAC)GATCCGCCAAGAAACTGCAAGAACGGATGGG NGG 10 8 17 8 24 0 100 5 10
    240604205022_k5xz TTTCCCCCATCCGTTCTTGCAGTTTCTTGGCG(+ACTCAC)GATCCTGCGAGTCGCAGGCAGACCCTCGGGG NGG 10 8 17 8 24 0 100 5 10
    240604205021_hm90 AAAAAAAAAAAGACTTTGAAAAGAAAGATCTG(+ACTCAC)GTACCAGGGCAGGCTTGGTGCAATGCCCACG NGG 10 8 17 8 24 0 100 5 10
    240604205018_rnku ACGTACGTGGGCATTGCACCAAGCCTGCCCTG(+ACTCAC)GTACCAGATCTTTCTTTTCAAAGTCTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240604205016_8d2c GGTGTTGCACTCCAACACTTTTTACTATTCCG(+ACTCAC)GATCCCAGCAAAGGTACACATCAGCAGGAAA NGG 10 8 17 8 24 0 100 5 10
    240604205014_sjxc ATTGATTTCCTGCTGATGTGTACCTTTGCTGG(+ACTCAC)GATCCGGAATAGTAAAAAGTGTTGGAGTGCA NGG 10 8 17 8 24 0 100 5 10
    240604205013_aj6j CCTGACTTCCAGTTAAGATGGAAGATTAGAAG(+ACTCAC)GTACCTCTCCTTGTGACCCACTTAAGGTGAA NGG 10 8 17 8 24 0 100 5 10
    240604205011_ctim GCAGTTTCACCTTAAGTGGGTCACAAGGAGAG(+ACTCAC)GTACCTTCTAATCTTCCATCTTAACTGGAAG NGG 10 8 17 8 24 0 100 5 10
    240604205010_68lh AGCAAGGAAGGGCTAGCCACCATCTTGAGAAG(+ACTCAC)GTACCTGTTGTTCAAAACTAACTCACTCTGG NGG 10 8 17 8 24 0 100 5 10