Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604205009_rfwn ACCCCCCAGAGTGAGTTAGTTTTGAACAACAG(+ACTCAC)GTACCTTCTCAAGATGGTGGCTAGCCCTTCC NGG 10 8 17 8 24 0 100 5 10
    240604205007_jj3z TTTCCCTCAGTGATGAAGAGGCCCCACTGTGG(+ACTCAC)GTACCAACCAACCCTGATACATAAAGTCACA NGG 10 8 17 8 24 0 100 5 10
    240604205006_iscs TTCCCTGTGACTTTATGTATCAGGGTTGGTTG(+ACTCAC)GTACCCACAGTGGGGCCTCTTCATCACTGAG NGG 10 8 17 8 24 0 100 5 10
    240604205004_pjly ACTTCACAAGAAGCTCTCTGCTCTCTGCACTG(+ACTCAC)GTACCCAACTATTCAGCTAATCTTCACTTCC NGG 10 8 17 8 24 0 100 5 10
    240604205002_0vjx AGAGAGGAAGTGAAGATTAGCTGAATAGTTGG(+ACTCAC)GTACCAGTGCAGAGAGCAGAGAGCTTCTTGT NGG 10 8 17 8 24 0 100 5 10
    240604205001_ugb2 AGTGTAGGGGCTTTAGCATCTTGATTTTTCAG(+ACTCAC)GATCCGATTCTGCCCAATGGTAATTTGCTAA NGG 10 8 17 8 24 0 100 5 10
    240604205000_5i8x TCCTTTTAGCAAATTACCATTGGGCAGAATCG(+ACTCAC)GATCCTGAAAAATCAAGATGCTAAAGCCCCT NGG 10 8 17 8 24 0 100 5 10
    240604204958_u6kj GGTGTCAGCGTGTGGATGCTGATTATGGGGTG(+ACTCAC)GATCCCTGGATATGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240604204956_hdjo GGATGGACCATGTAGAGACTGCCATATCCAGG(+ACTCAC)GATCCACCCCATAATCAGCATCCACACGCTG NGG 10 8 17 8 24 0 100 5 10
    240604204955_e870 TTCTGGATATATGCCCAGGAGAGGTATTGCTG(+ACTCAC)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10