Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604204907_02wn TGAAGTTCACGAGTAGTCTTCCAGCCTCTCAG(+ACTCAC)GTACCAAATAGTTTATGTATCAGGATAACAG NGG 10 8 17 8 24 0 100 5 10
    240604204905_elir ACTCTCTTCAGAGACACGAGAAGAGGGCATTG(+ACTCAC)GATCCCATTACAGATGGTTGTGAGTCACCAT NGG 10 8 17 8 24 0 100 5 10
    240604204904_z0zn ACCACATGGTGACTCACAACCATCTGTAATGG(+ACTCAC)GATCCAATGCCCTCTTCTCGTGTCTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240604204901_wdby CACTCACACACGCCTGTCTGGGGAGTCCCGAG(+ACTCAC)GATCCCATGGATTCCACACGGCCAGGGGCTG NGG 10 8 17 8 24 0 100 5 10
    240604204859_su6w GGCCTCAGCCCCTGGCCGTGTGGAATCCATGG(+ACTCAC)GATCCTCGGGACTCCCCAGACAGGCGTGTGT NGG 10 8 17 8 24 0 100 5 10
    240604204857_vqh6 CTGACCTTGGCTTGCCAATCGGTGAACTGAGG(+ACTCAC)GATCCCTTCAGAGCAGGTCCCAGCCAACCCA NGG 10 8 17 8 24 0 100 5 10
    240604204856_lj2j ATTGTTGGGTTGGCTGGGACCTGCTCTGAAGG(+ACTCAC)GATCCCTCAGTTCACCGATTGGCAAGCCAAG NGG 10 8 17 8 24 0 100 5 10
    240604204854_1v36 GAAAGTTTTTAAGCCACCAGCTGAGATGAGAG(+ACTCAC)GTACCTTTAAGCCTAGCCCACCATCACATCG NGG 10 8 17 8 24 0 100 5 10
    240604204853_fs6i CTCACCGATGTGATGGTGGGCTAGGCTTAAAG(+ACTCAC)GTACCTCTCATCTCAGCTGGTGGCTTAAAAA NGG 10 8 17 8 24 0 100 5 10
    240604204851_u331 CCTCAGGGTTAGTAAGGCAGGAGCTGCTAGTG(+ACTCAC)GTACCACAGGAACAGCAATGGCTGACATTAA NGG 10 8 17 8 24 0 100 5 10