Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604204829_csrl TTCCTCTGGAGCCCTTTCCAGAATGGCTTTGG(+ACTCAC)GATCCCCCCACCAAAGACCTGGTTGGGTGGC NGG 10 8 17 8 24 0 100 5 10
    240604204828_zzdx TCCTGTGCCTGCCTTCTCTTTCTGCCTGTATG(+ACTCAC)GATCCCCAAAGGCAGTGAGTTCCTGGCTTGT NGG 10 8 17 8 24 0 100 5 10
    240604204826_3hp3 AAAGGACAAGCCAGGAACTCACTGCCTTTGGG(+ACTCAC)GATCCATACAGGCAGAAAGAGAAGGCAGGCA NGG 10 8 17 8 24 0 100 5 10
    240604204823_ivut CATATAAGAACAAACAACCAAAATTTTCCCAG(+ACTCAC)GATCCCAGAGCCAGAGGCTACAAGAACAACT NGG 10 8 17 8 24 0 100 5 10
    240604204821_now1 CAAAAAGTTGTTCTTGTAGCCTCTGGCTCTGG(+ACTCAC)GATCCTGGGAAAATTTTGGTTGTTTGTTCTT NGG 10 8 17 8 24 0 100 5 10
    240604204819_k8c6 GCTTCTCAGCCCCACCCTCACCCTCTGGCCAG(+ACTCAC)GATCCTACAAGGCTACATCCCCTGCTGCTTG NGG 10 8 17 8 24 0 100 5 10
    240604204818_i9hm TCAACCAAGCAGCAGGGGATGTAGCCTTGTAG(+ACTCAC)GATCCTGGCCAGAGGGTGAGGGTGGGGCTGA NGG 10 8 17 8 24 0 100 5 10
    240604204816_4rle CAGGCTGCTGCCCCTCTGCAGCTCTACCAGAG(+ACTCAC)GTACCCGGCCTACTCTGCTGGAAAACTAGGT NGG 10 8 17 8 24 0 100 5 10
    240604204814_sck3 CCCAGACCTAGTTTTCCAGCAGAGTAGGCCGG(+ACTCAC)GTACCTCTGGTAGAGCTGCAGAGGGGCAGCA NGG 10 8 17 8 24 0 100 5 10
    240604204812_dpem CAGTCTCCTCTACCCTCTGAACCACCTCCTCG(+ACTCAC)GATCCCATAGGCTACATTTTTTTTCCTTCCA NGG 10 8 17 8 24 0 100 5 10