Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604205210_kzdt GTTCCACTTAGTCAGGAAGTCTCCAGCCTATG(+ACTCAC)GATCCTGTGCCACCCCCTGCCCCCAACGCCC NGG 10 8 17 8 24 0 100 5 10
    240604205208_oj5g TGTTTGGGCGTTGGGGGCAGGGGGTGGCACAG(+ACTCAC)GATCCATAGGCTGGAGACTTCCTGACTAAGT NGG 10 8 17 8 24 0 100 5 10
    240604205207_60r6 TGAGATGAGTCCACCTCATCTGTAGCCTAGAG(+ACTCAC)GTACCTCAGTGAGTTGGGAAAGTCAGAGGCC NGG 10 8 17 8 24 0 100 5 10
    240604205206_vpi2 TAAGTGGCCTCTGACTTTCCCAACTCACTGAG(+ACTCAC)GTACCTCTAGGCTACAGATGAGGTGGACTCA NGG 10 8 17 8 24 0 100 5 10
    240604205204_a8qx TCGCTGGGGCTGCCCCATAGGACACTGGGGTG(+ACTCAC)GTACCATGGAGAGACAGGGGATAAAACTGAT NGG 10 8 17 8 24 0 100 5 10
    240604205202_nsnh TTTGGATCAGTTTTATCCCCTGTCTCTCCATG(+ACTCAC)GTACCACCCCAGTGTCCTATGGGGCAGCCCC NGG 10 8 17 8 24 0 100 5 10
    240604205201_5i5t GCTCAAAATGTCTCTGGCTGTATTGATCTGAG(+ACTCAC)GATCCTCCCCTCTTTTGAACCATTCCTTCCA NGG 10 8 17 8 24 0 100 5 10
    240604205159_x3v8 GGTGATGGAAGGAATGGTTCAAAAGAGGGGAG(+ACTCAC)GATCCTCAGATCAATACAGCCAGAGACATTT NGG 10 8 17 8 24 0 100 5 10
    240604205158_ug8q CAAGTCTGTGCCTCAGCACAGCTAGAAGTCAG(+ACTCAC)GATCCAAGGAGGCCTAAAATCGGCAATGACA NGG 10 8 17 8 24 0 100 5 10
    240604205155_p3y8 GAGTCTGTCATTGCCGATTTTAGGCCTCCTTG(+ACTCAC)GATCCTGACTTCTAGCTGTGCTGAGGCACAG NGG 10 8 17 8 24 0 100 5 10