Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604204610_qyic GGCTTCTTTGACTGTCAAGTGATTAACATATG(+ACTCAC)GTACCCGGTGTCAGAACATTTAAGCGTTCTA NGG 10 8 17 8 24 0 100 5 10
    240604204608_s6sj TCTTAAAATACAAGCAGGTATCTCATCTGAAG(+ACTCAC)GTACCCTCACAGCTTCCTCGCAACAGCAGGG NGG 10 8 17 8 24 0 100 5 10
    240604204607_hwt0 CCTGTCCCTGCTGTTGCGAGGAAGCTGTGAGG(+ACTCAC)GTACCTTCAGATGAGATACCTGCTTGTATTT NGG 10 8 17 8 24 0 100 5 10
    240604204605_0a4e CCTGATGCTACAGAAGCCAGATGAGGGGACTG(+ACTCAC)GATCCCCTGGAACTGGAGGTACAGATGGCTG NGG 10 8 17 8 24 0 100 5 10
    240604204603_cjgi GCTCACAGCCATCTGTACCTCCAGTTCCAGGG(+ACTCAC)GATCCAGTCCCCTCATCTGGCTTCTGTAGCA NGG 10 8 17 8 24 0 100 5 10
    240604204601_iiyc GCCACGCCTCGCTCTCTGTGTACAGCAATGGG(+ACTCAC)GATCCAGGAAGCAGGATGGGCCAGGAACCCG NGG 10 8 17 8 24 0 100 5 10
    240604204559_0wr2 GCCTTCGGGTTCCTGGCCCATCCTGCTTCCTG(+ACTCAC)GATCCCCATTGCTGTACACAGAGAGCGAGGC NGG 10 8 17 8 24 0 100 5 10
    240604204558_zgi5 AGACTATGTGCTACCTTCACTTGCTAATGCAG(+ACTCAC)GATCCAAAGAATGCAGGCAAACCCTACAAAT NGG 10 8 17 8 24 0 100 5 10
    240604204557_up33 GCTACATTTGTAGGGTTTGCCTGCATTCTTTG(+ACTCAC)GATCCTGCATTAGCAAGTGAAGGTAGCACAT NGG 10 8 17 8 24 0 100 5 10
    240604204556_26r3 TAAATGGGACAAAGACATAGGCAAAAAACATG(+ACTCAC)GATCCTAAGTAGAATCAGATATTTATCTCTT NGG 10 8 17 8 24 0 100 5 10