Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604204554_moua AGAAAAAGAGATAAATATCTGATTCTACTTAG(+ACTCAC)GATCCATGTTTTTTGCCTATGTCTTTGTCCC NGG 10 8 17 8 24 0 100 5 10
    240604204553_55mk ATACTTTCCACATCTGGGGAATGTAAGCTAGG(+ACTCAC)GTACCTCATCTGGGATTTATCTTATTCTTCC NGG 10 8 17 8 24 0 100 5 10
    240604204552_vlz2 GATCTGGAAGAATAAGATAAATCCCAGATGAG(+ACTCAC)GTACCCTAGCTTACATTCCCCAGATGTGGAA NGG 10 8 17 8 24 0 100 5 10
    240604204550_xija AGTCCACAGGATAAAAGAATGTGACCCCAAAG(+ACTCAC)GATCCTTCTGTAGCTGATCAAGCCACCCTAA NGG 10 8 17 8 24 0 100 5 10
    240604204548_gjnz ATTAATTAGGGTGGCTTGATCAGCTACAGAAG(+ACTCAC)GATCCTTTGGGGTCACATTCTTTTATCCTGT NGG 10 8 17 8 24 0 100 5 10
    240604204547_f29p TGTGCCACAGGATTATGCATAGCCACCGTTAG(+ACTCAC)GTACCACAGCTGTTACACAGAGCTTTAAACT NGG 10 8 17 8 24 0 100 5 10
    240604204546_slp1 CAATCAGTTTAAAGCTCTGTGTAACAGCTGTG(+ACTCAC)GTACCTAACGGTGGCTATGCATAATCCTGTG NGG 10 8 17 8 24 0 100 5 10
    240604204545_wy2k ATTGAAGTTTTTTTCCATATAAAGATAGATAG(+ACTCAC)GTACCATAAAATCAAGTGACTGCTTTTATAA NGG 10 8 17 8 24 0 100 5 10
    240604204543_lf2j CTCTGTTATAAAAGCAGTCACTTGATTTTATG(+ACTCAC)GTACCTATCTATCTTTATATGGAAAAAAACT NGG 10 8 17 8 24 0 100 5 10
    240604204541_d7m0 AACATATACCACATGACAAGACAAACTGAGGG(+ACTCAC)GATCCTAGTAAAGACCAGAGGTTACTTAAAA NGG 10 8 17 8 24 0 100 5 10