Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604204540_70eh ATTGCTTTTAAGTAACCTCTGGTCTTTACTAG(+ACTCAC)GATCCCCTCAGTTTGTCTTGTCATGTGGTAT NGG 10 8 17 8 24 0 100 5 10
    240604204539_1wtw AAAAGGAAAAAGAATTAGACTGGATAGAAGTG(+ACTCAC)GTACCCAACACTGGGACTCAATTTTTATTTT NGG 10 8 17 8 24 0 100 5 10
    240604204537_btd2 AATTTAAAATAAAAATTGAGTCCCAGTGTTGG(+ACTCAC)GTACCACTTCTATCCAGTCTAATTCTTTTTC NGG 10 8 17 8 24 0 100 5 10
    240604204536_hzem AGATTTCAAGTAAAACTGAGCTGAGATACAAG(+ACTCAC)GATCCCCAAAAAAGTTCTTAGCTTATAAAAC NGG 10 8 17 8 24 0 100 5 10
    240604204534_2ojy ACTTAGTTTTATAAGCTAAGAACTTTTTTGGG(+ACTCAC)GATCCTTGTATCTCAGCTCAGTTTTACTTGA NGG 10 8 17 8 24 0 100 5 10
    240604204532_9am2 AGTAGCCATTCTCTAAATGAACTGTAGGAGTG(+ACTCAC)GATCCTTAGGGAAGGGAGCGCAGGTCATTCA NGG 10 8 17 8 24 0 100 5 10
    240604204531_wi4n AGTTCTGAATGACCTGCGCTCCCTTCCCTAAG(+ACTCAC)GATCCACTCCTACAGTTCATTTAGAGAATGG NGG 10 8 17 8 24 0 100 5 10
    240604204529_z2f5 ACAAGAAACCCCTGTCTAACGCAGGGCCTCAG(+ACTCAC)GATCCGAGACGGCTTTGGAAGTGAACTGCAA NGG 10 8 17 8 24 0 100 5 10
    240604204527_v5wd TGGCATTGCAGTTCACTTCCAAAGCCGTCTCG(+ACTCAC)GATCCTGAGGCCCTGCGTTAGACAGGGGTTT NGG 10 8 17 8 24 0 100 5 10
    240604204525_5598 ACAAGAAACCCCTGTCTAACGCAGGGCCTCAG(+ACTCAC)GATCCGAGACGGCTTTGGAAGTGAACTGCAA NGG 10 8 17 8 24 0 100 5 10