Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604204504_yxun CCACACACCCTGCACAGTGCTGCCCCGGGGAG(+ACTCAC)GTACCAAAGCCTACACCTACAGAGACTTGTG NGG 10 8 17 8 24 0 100 5 10
    240604204501_5jn5 CTGTTTCCCTAAACTCCATCCTTTCTTCCCAG(+ACTCAC)GATCCTGCCCTTCATAGAGGGAACAGGGTAG NGG 10 8 17 8 24 0 100 5 10
    240604204500_18zc TGGAGCTACCCTGTTCCCTCTATGAAGGGCAG(+ACTCAC)GATCCTGGGAAGAAAGGATGGAGTTTAGGGA NGG 10 8 17 8 24 0 100 5 10
    240604204458_f2hv CTGGGGCTTCTCTCCACTTTCTGGCAAGGAAG(+ACTCAC)GTACCAGGGTCACAGACTCCCACACAGTCTC NGG 10 8 17 8 24 0 100 5 10
    240604204455_bg1e TACTTGAGACTGTGTGGGAGTCTGTGACCCTG(+ACTCAC)GTACCTTCCTTGCCAGAAAGTGGAGAGAAGC NGG 10 8 17 8 24 0 100 5 10
    240604204453_1uoc CCTGTCAGTGAGGGGGGTTGACTCCTGTGTGG(+ACTCAC)GTACCATTGGGGAAGGATGGATTTATCACAC NGG 10 8 17 8 24 0 100 5 10
    240604204451_wayg GGAGAGTGTGATAAATCCATCCTTCCCCAATG(+ACTCAC)GTACCCACACAGGAGTCAACCCCCCTCACTG NGG 10 8 17 8 24 0 100 5 10
    240604204450_7qro AGACCAAGAACAGCAGAAGCTCACAGAGTGAG(+ACTCAC)GATCCAGGGAAGCCTTGGCAGGCTGTGGGCA NGG 10 8 17 8 24 0 100 5 10
    240604204447_xypm CCGCTTGCCCACAGCCTGCCAAGGCTTCCCTG(+ACTCAC)GATCCTCACTCTGTGAGCTTCTGCTGTTCTT NGG 10 8 17 8 24 0 100 5 10
    240604204445_rexd CACCTGGGAACCACTCGACTACTGACCCAAAG(+ACTCAC)GTACCCTCCCCAGGGCTGCTCAGGACCAGTG NGG 10 8 17 8 24 0 100 5 10