Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604204351_3dsu GATGACCTGACCCTCCAGGTAGCATCTGACAG(+ACTCAC)GTACCCGACTCCTTTTCTTGGGCAAGCCTCT NGG 10 8 17 8 24 0 100 5 10
    240604204349_zw2f AGGTTGGGATGTCCCTGTTGCTAGCCCATTAG(+ACTCAC)GATCCTAGCTCCAATTCTGACCTTGAAAAAG NGG 10 8 17 8 24 0 100 5 10
    240604204348_csuh CTTGACTTTTTCAAGGTCAGAATTGGAGCTAG(+ACTCAC)GATCCTAATGGGCTAGCAACAGGGACATCCC NGG 10 8 17 8 24 0 100 5 10
    240604204346_00yw CTCCATGATGGTGGGACACCTGCTCCCGTGTG(+ACTCAC)GATCCTATAGCTAGTGTCCTTCAGTCCACTG NGG 10 8 17 8 24 0 100 5 10
    240604204345_xgf0 GCACGCAGTGGACTGAAGGACACTAGCTATAG(+ACTCAC)GATCCACACGGGAGCAGGTGTCCCACCATCA NGG 10 8 17 8 24 0 100 5 10
    240604204344_llgy GGGTTTTTCTAGTCAGTGAGATACTCCAACTG(+ACTCAC)GATCCCAAAGATGGAGTACCTTAGCGTCACT NGG 10 8 17 8 24 0 100 5 10
    240604204341_g4zi AGTTCAGTGACGCTAAGGTACTCCATCTTTGG(+ACTCAC)GATCCAGTTGGAGTATCTCACTGACTAGAAA NGG 10 8 17 8 24 0 100 5 10
    240604204340_6va9 TGGTGGGAGTTACGGCTGCTGGTGGGAGTCTG(+ACTCAC)GTACCTTTCTGGTTGAGCAATGCAGCAAAAT NGG 10 8 17 8 24 0 100 5 10
    240604204338_9bro GTGGAATTTTGCTGCATTGCTCAACCAGAAAG(+ACTCAC)GTACCAGACTCCCACCAGCAGCCGTAACTCC NGG 10 8 17 8 24 0 100 5 10
    240604204336_ljh8 GATGCAGGGCTGGAAGGAGCCAAGCAAGGAGG(+ACTCAC)GTACCCAGGAGGAGCTGGCAGGGCGAGAACA NGG 10 8 17 8 24 0 100 5 10