Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604204319_dn2a TCAAACTCTGGGCTCAGTTCTCAGGCCACAGG(+ACTCAC)GTACCCAGCCTGCACCTAGCAAGGTTGCTGA NGG 10 8 17 8 24 0 100 5 10
    240604204317_1j7r TCCCGCTCTGGGACTTACTGGGGAGGGGGCGG(+ACTCAC)GTACCAGTGTATGGAGTAACAAGACATCAGC NGG 10 8 17 8 24 0 100 5 10
    240604204316_fu6b CGAAGGCTGATGTCTTGTTACTCCATACACTG(+ACTCAC)GTACCCGCCCCCTCCCCAGTAAGTCCCAGAG NGG 10 8 17 8 24 0 100 5 10
    240604204314_bmax GGCTTGCCTGTCGGTCAAGAGACCCTCGGATG(+ACTCAC)GATCCCAGGACTGTAAAATATAAATAAATAA NGG 10 8 17 8 24 0 100 5 10
    240604204312_6f73 TATTTTTATTTATTTATATTTTACAGTCCTGG(+ACTCAC)GATCCATCCGAGGGTCTCTTGACCGACAGGC NGG 10 8 17 8 24 0 100 5 10
    240604204310_ad86 CAATGCTGTTCGCTAGATTTTGCAACCACCTG(+ACTCAC)GATCCTGTTTGCCGATACCCCTCATCCGGAT NGG 10 8 17 8 24 0 100 5 10
    240604204309_unel CAGAAATCCGGATGAGGGGTATCGGCAAACAG(+ACTCAC)GATCCAGGTGGTTGCAAAATCTAGCGAACAG NGG 10 8 17 8 24 0 100 5 10
    240604204307_u7a4 CTGTGTCTCATATTTATATCATAAAAACATTG(+ACTCAC)GATCCCTATAACTTCAAAGTCGTGTGCTTCT NGG 10 8 17 8 24 0 100 5 10
    240604204306_wfcw AGTGAAGAAGCACACGACTTTGAAGTTATAGG(+ACTCAC)GATCCAATGTTTTTATGATATAAATATGAGA NGG 10 8 17 8 24 0 100 5 10
    240604204305_x68i AGGTTTCTAATTGCCCAGCTGCTAGGTAACTG(+ACTCAC)GTACCTTTTAAAGCCCCCTGTAATTTTCCTT NGG 10 8 17 8 24 0 100 5 10