Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604204232_rgcf TGTTAATTTTCAGTGTTGTTGGATTCTTGGTG(+ACTCAC)GTACCTTGTGTTTTCATAATTATGTCTTCAT NGG 10 8 17 8 24 0 100 5 10
    240604204230_j1cb AAATTACATAAACTACTGGAAAACCAGTGAAG(+ACTCAC)GTACCAGGTCAATAGATTAAAAGACTATACA NGG 10 8 17 8 24 0 100 5 10
    240604204228_v9y5 CGTATTGTATAGTCTTTTAATCTATTGACCTG(+ACTCAC)GTACCTTCACTGGTTTTCCAGTAGTTTATGT NGG 10 8 17 8 24 0 100 5 10
    240604204226_g2te CCTTAGGCAGTGCCTGCTACTCCAAAGGGGAG(+ACTCAC)GTACCGTTAGTGTGCAGGAATGAGACACTTG NGG 10 8 17 8 24 0 100 5 10
    240604204225_4gvh CCTTCCAAGTGTCTCATTCCTGCACACTAACG(+ACTCAC)GTACCTCCCCTTTGGAGTAGCAGGCACTGCC NGG 10 8 17 8 24 0 100 5 10
    240604204223_zbvw GTGCAGGAATGAGACACTTGGAAGGCAGATGG(+ACTCAC)GATCCAAGAGAAAGGCATACTTGGTAAACTA NGG 10 8 17 8 24 0 100 5 10
    240604204222_v7yg CAATATAGTTTACCAAGTATGCCTTTCTCTTG(+ACTCAC)GATCCCATCTGCCTTCCAAGTGTCTCATTCC NGG 10 8 17 8 24 0 100 5 10
    240604204221_s7n6 CATCTACTTTGCCTTTTTGTGCAGGTTATATG(+ACTCAC)GATCCTGCACCGCTCATTACAAAGGTCTGCA NGG 10 8 17 8 24 0 100 5 10
    240604204219_sy9e AAACGTGCAGACCTTTGTAATGAGCGGTGCAG(+ACTCAC)GATCCATATAACCTGCACAAAAAGGCAAAGT NGG 10 8 17 8 24 0 100 5 10
    240604204218_bhqe GAGTCCAGCATAACCAGAAAGTCCATGAGCTG(+ACTCAC)GTACCCCTGAAGCCTGGGTGTGCAGACGAGC NGG 10 8 17 8 24 0 100 5 10