Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604204631_0vno CTCCTCTGGTCTCCATTGGCACCAGGCACCAG(+ACTCAC)GTACCAGGCACAAATGTGGTGTACAGACAGA NGG 10 8 17 8 24 0 100 5 10
    240604204630_vhx8 GACAAAGAAGCGGGACTGCTTCGTTCTTCTAG(+ACTCAC)GTACCGGATGGGGTCTTTTGTTTACAGTAAA NGG 10 8 17 8 24 0 100 5 10
    240604204628_3npa ATAGCTTTACTGTAAACAAAAGACCCCATCCG(+ACTCAC)GTACCTAGAAGAACGAAGCAGTCCCGCTTCT NGG 10 8 17 8 24 0 100 5 10
    240604204627_sk3n GTGAATAGGAAAATGGAGGTTATGATGGTTTG(+ACTCAC)GTACCAAGAGAATAATAAATGTCTGACTAAT NGG 10 8 17 8 24 0 100 5 10
    240604204626_ab8s AATTCATTAGTCAGACATTTATTATTCTCTTG(+ACTCAC)GTACCAAACCATCATAACCTCCATTTTCCTA NGG 10 8 17 8 24 0 100 5 10
    240604204625_njpq TCAGGCCATCAGGCTTGGCTATATGGCAGAAG(+ACTCAC)GTACCTTTACCCACTGAACCACCTCGGCAGC NGG 10 8 17 8 24 0 100 5 10
    240604204624_wllf GAGAGGCTGCCGAGGTGGTTCAGTGGGTAAAG(+ACTCAC)GTACCTTCTGCCATATAGCCAAGCCTGATGG NGG 10 8 17 8 24 0 100 5 10
    240604204621_orcg CCTCCACCATTACACACAGGGTGAGGCCCTAG(+ACTCAC)GATCCACAGAGTTGCCTGGAGGCTGCACGGT NGG 10 8 17 8 24 0 100 5 10
    240604204620_2wzh TGTGAACCGTGCAGCCTCCAGGCAACTCTGTG(+ACTCAC)GATCCTAGGGCCTCACCCTGTGTGTAATGGT NGG 10 8 17 8 24 0 100 5 10
    240604204618_y0lv GAAGACCTAGGCATAGCATATCAAACACATTG(+ACTCAC)GATCCCACGATGTTAGATATAGCCATGTCTC NGG 10 8 17 8 24 0 100 5 10