Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604204115_35dz CGCCATATTCAGGTCTAAGTGCCTGCTCTCGG(+ACTCAC)GTACCGTGAGCAAACTTGCCCTTTTATGAAG NGG 10 8 17 8 24 0 100 5 10
    240604204114_4d9n CCAGCTACTGCATCCAGTGGCCTGGGGCTCAG(+ACTCAC)GATCCATTTTCTACATTCATAAGAATGCCCA NGG 10 8 17 8 24 0 100 5 10
    240604204113_hgu8 GGGCATGGGCATTCTTATGAATGTAGAAAATG(+ACTCAC)GATCCTGAGCCCCAGGCCACTGGATGCAGTA NGG 10 8 17 8 24 0 100 5 10
    240604204111_2i3d TATAGGACTTGATGAAATTAAGCAAGGTCCTG(+ACTCAC)GATCCCAGAAACAGGTCCACAGGTTCTTCGG NGG 10 8 17 8 24 0 100 5 10
    240604204109_a57c GCATACCGAAGAACCTGTGGACCTGTTTCTGG(+ACTCAC)GATCCAGGACCTTGCTTAATTTCATCAAGTC NGG 10 8 17 8 24 0 100 5 10
    240604204108_yy1m CGATGGCTCTATTAATACACACACGGTGACAG(+ACTCAC)GTACCCTATTTAGACTAAGGCTACTAGAACA NGG 10 8 17 8 24 0 100 5 10
    240604204106_76j1 GAGTGTGTTCTAGTAGCCTTAGTCTAAATAGG(+ACTCAC)GTACCTGTCACCGTGTGTGTATTAATAGAGC NGG 10 8 17 8 24 0 100 5 10
    240604204104_teby GATACATCTACTCAGCATAGGAGAGCCCTGGG(+ACTCAC)GTACCACTTACAGGCTATCTAGAATAGTTCC NGG 10 8 17 8 24 0 100 5 10
    240604204102_mntx GCTGTGGAACTATTCTAGATAGCCTGTAAGTG(+ACTCAC)GTACCCCAGGGCTCTCCTATGCTGAGTAGAT NGG 10 8 17 8 24 0 100 5 10
    240604204101_mq2w CATGTGTGCTCGTTGTCTTAAGTCCCTGGAGG(+ACTCAC)GTACCACACTGCTTTCATCCTGTCCCCTTGG NGG 10 8 17 8 24 0 100 5 10