Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604204201_vubb TCATAATGTACCGATACTATTGGAATATTGGG(+ACTCAC)GTACCTTTTCCTTCTGAGTATCTATAAGTGA NGG 10 8 17 8 24 0 100 5 10
    240604204159_2mu5 ATCAAGTTTCTTTTCAGCAGGAAAGCCAAGAG(+ACTCAC)GTACCTTTTGTCCACATCAAATGTCCCTTGC NGG 10 8 17 8 24 0 100 5 10
    240604204158_w5nk ATTCTGCAAGGGACATTTGATGTGGACAAAAG(+ACTCAC)GTACCTCTTGGCTTTCCTGCTGAAAAGAAAC NGG 10 8 17 8 24 0 100 5 10
    240604204157_o3x5 ATGACTGCTTTGACTGCTTATAAAAGTGACAG(+ACTCAC)GATCCTCAGTAAGCAGCAACTCGAGAACTTG NGG 10 8 17 8 24 0 100 5 10
    240604204155_l6nj GTCCCCAAGTTCTCGAGTTGCTGCTTACTGAG(+ACTCAC)GATCCTGTCACTTTTATAAGCAGTCAAAGCA NGG 10 8 17 8 24 0 100 5 10
    240604204154_imvq GATAACTTCACAACATTGGAGTGGTCAGAAAG(+ACTCAC)GTACCTGTTAAATGTTACTAACGAAACAAAT NGG 10 8 17 8 24 0 100 5 10
    240604204153_ehc1 AGAGCATTTGTTTCGTTAGTAACATTTAACAG(+ACTCAC)GTACCTTTCTGACCACTCCAATGTTGTGAAG NGG 10 8 17 8 24 0 100 5 10
    240604204151_p1fx TTCCTCTATAAATTTTAAGTCTTGTTGCAGAG(+ACTCAC)GTACCCAGGAAGTCCTGCTGTCCTCTAATGG NGG 10 8 17 8 24 0 100 5 10
    240604204149_mnsi ACAACCCATTAGAGGACAGCAGGACTTCCTGG(+ACTCAC)GTACCTCTGCAACAAGACTTAAAATTTATAG NGG 10 8 17 8 24 0 100 5 10
    240604204148_oo3i ACCGTGGTTTCTGCTTCAGCCCCTGCATCTAG(+ACTCAC)GATCCTGCTGTGTTTGAGATCTTGTCCTGAC NGG 10 8 17 8 24 0 100 5 10