Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604204407_xcu4 GGAATGCTGGTGCTCAGGTTGGAGAAGCTAGG(+ACTCAC)GATCCCAGGGGCAGCTGCCTGGTTAGGTTGC NGG 10 8 17 8 24 0 100 5 10
    240604204405_mi4v GAGCCCTTTATTGGCTTGGATTAGCTGTGTAG(+ACTCAC)GATCCAGGGACTGTCCTCGTCCTCGGGTTGG NGG 10 8 17 8 24 0 100 5 10
    240604204403_xj9j GCAAGCCAACCCGAGGACGAGGACAGTCCCTG(+ACTCAC)GATCCTACACAGCTAATCCAAGCCAATAAAG NGG 10 8 17 8 24 0 100 5 10
    240604204401_tmt8 AATGTCTCCTCTCCAGTAGCTCCACAGAGGTG(+ACTCAC)GTACCTCAGCCAAAGCCATTCCTAACCCATG NGG 10 8 17 8 24 0 100 5 10
    240604204400_7u5x AGGTTCATGGGTTAGGAATGGCTTTGGCTGAG(+ACTCAC)GTACCACCTCTGTGGAGCTACTGGAGAGGAG NGG 10 8 17 8 24 0 100 5 10
    240604204359_ljmy AAAAAGCTTTATTAAAAGTTAGCCCTTACAAG(+ACTCAC)GATCCTATTAAAATATACACATTTTAAATAA NGG 10 8 17 8 24 0 100 5 10
    240604204357_1t4c TGCATTTATTTAAAATGTGTATATTTTAATAG(+ACTCAC)GATCCTTGTAAGGGCTAACTTTTAATAAAGC NGG 10 8 17 8 24 0 100 5 10
    240604204356_5hiz TCTAATCTCTGTCAAAAGGGAGGTTAGGAAAG(+ACTCAC)GTACCCCCTCCCTTGTAAACACTGAAATACA NGG 10 8 17 8 24 0 100 5 10
    240604204353_0y1p AGATCTGTATTTCAGTGTTTACAAGGGAGGGG(+ACTCAC)GTACCTTTCCTAACCTCCCTTTTGACAGAGA NGG 10 8 17 8 24 0 100 5 10
    240604204352_zfnd CTGCCAGAGGCTTGCCCAAGAAAAGGAGTCGG(+ACTCAC)GTACCTGTCAGATGCTACCTGGAGGGTCAGG NGG 10 8 17 8 24 0 100 5 10