Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604204849_0cew TGAAGTTAATGTCAGCCATTGCTGTTCCTGTG(+ACTCAC)GTACCACTAGCAGCTCCTGCCTTACTAACCC NGG 10 8 17 8 24 0 100 5 10
    240604204847_zphf GAGGTGGGGTGCTTTATAGGATTCTGCCTTCG(+ACTCAC)GATCCAAAACTCTGACCAGGGTGGCTGCTCC NGG 10 8 17 8 24 0 100 5 10
    240604204845_5r7p GAGATGGAGCAGCCACCCTGGTCAGAGTTTTG(+ACTCAC)GATCCGAAGGCAGAATCCTATAAAGCACCCC NGG 10 8 17 8 24 0 100 5 10
    240604204843_ppyw CATCTCTATAACCGTTTGCTCTTGGACTCCCG(+ACTCAC)GATCCCTGTACCCCAGGTTGTTTTCCCTGTA NGG 10 8 17 8 24 0 100 5 10
    240604204842_t2r3 CAGGTTACAGGGAAAACAACCTGGGGTACAGG(+ACTCAC)GATCCGGGAGTCCAAGAGCAAACGGTTATAG NGG 10 8 17 8 24 0 100 5 10
    240604204840_o7eg CATCTCTATAACCGTTTGCTCTTGGACTCCCG(+ACTCAC)GATCCCTGTACCCCAGGTTGTTTTCCCTGTA NGG 10 8 17 8 24 0 100 5 10
    240604204837_qsve CAGGTTACAGGGAAAACAACCTGGGGTACAGG(+ACTCAC)GATCCGGGAGTCCAAGAGCAAACGGTTATAG NGG 10 8 17 8 24 0 100 5 10
    240604204835_4z45 ATGAGCAAAGGCAGGGACCGGGGCTTGCTAGG(+ACTCAC)GTACCAGCCCAGTTCTCCAGCCGTGAAGTGG NGG 10 8 17 8 24 0 100 5 10
    240604204833_a8ie GAGACCCACTTCACGGCTGGAGAACTGGGCTG(+ACTCAC)GTACCCTAGCAAGCCCCGGTCCCTGCCTTTG NGG 10 8 17 8 24 0 100 5 10
    240604204831_znep CCCCTGCCACCCAACCAGGTCTTTGGTGGGGG(+ACTCAC)GATCCCAAAGCCATTCTGGAAAGGGCTCCAG NGG 10 8 17 8 24 0 100 5 10