Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604203919_mgok GGTAGCTCACCAAGAGCTAGAACTTTTGCTTG(+ACTCAC)GTACCCAAAGGTTTTATTTGGGATTAGCCCG NGG 10 8 17 8 24 0 100 5 10
    240604203917_p1kx GCCTCCGGGCTAATCCCAAATAAAACCTTTGG(+ACTCAC)GTACCAAGCAAAAGTTCTAGCTCTTGGTGAG NGG 10 8 17 8 24 0 100 5 10
    240604203915_kd6l GGTAGCTCACCAAGAGCTAGAACTTTTGCTTG(+ACTCAC)GTACCCAAAGGTTTTATTTGGGATTAGCCCG NGG 10 8 17 8 24 0 100 5 10
    240604203914_u4bw ACCCCAGGAACTCCTAAACAGTTTAGGAGCTG(+ACTCAC)GATCCTCCCACTCTGGGATGACCCAAATGTT NGG 10 8 17 8 24 0 100 5 10
    240604203912_b2ft TCGGGAACATTTGGGTCATCCCAGAGTGGGAG(+ACTCAC)GATCCAGCTCCTAAACTGTTTAGGAGTTCCT NGG 10 8 17 8 24 0 100 5 10
    240604203910_i2n3 CATCAGGACAAGATTTGTATAGGAATGCTGGG(+ACTCAC)GATCCAAACTCAAGTGTACTTATACCAACCA NGG 10 8 17 8 24 0 100 5 10
    240604203908_ce32 GGACTTGGTTGGTATAAGTACACTTGAGTTTG(+ACTCAC)GATCCCCAGCATTCCTATACAAATCTTGTCC NGG 10 8 17 8 24 0 100 5 10
    240604203907_vsj7 CAAACAGTTTAATAGCAAATAAACAAAGGAAG(+ACTCAC)GTACCACACTTGGCAGATACAAAATGACTTT NGG 10 8 17 8 24 0 100 5 10
    240604203905_owyw GACAAAAAGTCATTTTGTATCTGCCAAGTGTG(+ACTCAC)GTACCTTCCTTTGTTTATTTGCTATTAAACT NGG 10 8 17 8 24 0 100 5 10
    240604203904_8w7r TGTACTCCCTGAGCAGAGCAGCCAGCCTCCTG(+ACTCAC)GATCCCCCACTGAGACCTGGAGGCCACCACA NGG 10 8 17 8 24 0 100 5 10