Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604204341_g4zi AGTTCAGTGACGCTAAGGTACTCCATCTTTGG(+ACTCAC)GATCCAGTTGGAGTATCTCACTGACTAGAAA NGG 10 8 17 8 24 0 100 5 10
    240604204340_6va9 TGGTGGGAGTTACGGCTGCTGGTGGGAGTCTG(+ACTCAC)GTACCTTTCTGGTTGAGCAATGCAGCAAAAT NGG 10 8 17 8 24 0 100 5 10
    240604204338_9bro GTGGAATTTTGCTGCATTGCTCAACCAGAAAG(+ACTCAC)GTACCAGACTCCCACCAGCAGCCGTAACTCC NGG 10 8 17 8 24 0 100 5 10
    240604204336_ljh8 GATGCAGGGCTGGAAGGAGCCAAGCAAGGAGG(+ACTCAC)GTACCCAGGAGGAGCTGGCAGGGCGAGAACA NGG 10 8 17 8 24 0 100 5 10
    240604204334_6tsj CCAGGTGTTCTCGCCCTGCCAGCTCCTCCTGG(+ACTCAC)GTACCCTCCTTGCTTGGCTCCTTCCAGCCCT NGG 10 8 17 8 24 0 100 5 10
    240604204333_suvk CTCTACTCTTTCTTGCCATCGCCCCTGGCATG(+ACTCAC)GATCCAGAGACTCAGGCTACAACAGGAAGCC NGG 10 8 17 8 24 0 100 5 10
    240604204331_nww8 AAAGTGGCTTCCTGTTGTAGCCTGAGTCTCTG(+ACTCAC)GATCCATGCCAGGGGCGATGGCAAGAAAGAG NGG 10 8 17 8 24 0 100 5 10
    240604204330_hxrv AGCCAAGCTGCGTGAGTGGAGTGTGGGGAATG(+ACTCAC)GATCCCTTGGGGGCTACAGAAAGAGTGAAGG NGG 10 8 17 8 24 0 100 5 10
    240604204328_vd8k AAGAGCCTTCACTCTTTCTGTAGCCCCCAAGG(+ACTCAC)GATCCATTCCCCACACTCCACTCACGCAGCT NGG 10 8 17 8 24 0 100 5 10
    240604204327_17pk ACACACACAGAGACCTGCCCCTGCTGTGGTTG(+ACTCAC)GATCCAATAAACTTTCTGGAGGCATAGCTTG NGG 10 8 17 8 24 0 100 5 10