Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604203901_srt4 CTCTTTGTGGTGGCCTCCAGGTCTCAGTGGGG(+ACTCAC)GATCCAGGAGGCTGGCTGCTCTGCTCAGGGA NGG 10 8 17 8 24 0 100 5 10
    240604203900_gzb7 TCCCTGCATGTATGCCTGCAGGTCAGAAGAGG(+ACTCAC)GTACCAGATCCCGTTACAGATGGTTGTGAGC NGG 10 8 17 8 24 0 100 5 10
    240604203858_vtga TGGTGGCTCACAACCATCTGTAACGGGATCTG(+ACTCAC)GTACCCTCTTCTGACCTGCAGGCATACATGC NGG 10 8 17 8 24 0 100 5 10
    240604203857_ao2c AGTAGGCTAGTCTGGCTGGCCAGCACCACAGG(+ACTCAC)GATCCTGTCTCTACCCTCCAGCATGGGCATC NGG 10 8 17 8 24 0 100 5 10
    240604203855_3a27 CTTGTGATGCCCATGCTGGAGGGTAGAGACAG(+ACTCAC)GATCCCTGTGGTGCTGGCCAGCCAGACTAGC NGG 10 8 17 8 24 0 100 5 10
    240604203854_l7mt CCTGGCAGACCCTAAGGGTTTTAGGCAGCTGG(+ACTCAC)GATCCAGTGGGGAGGGCTGGCAAAGGAAAGG NGG 10 8 17 8 24 0 100 5 10
    240604203852_qgve TAATTCCTTTCCTTTGCCAGCCCTCCCCACTG(+ACTCAC)GATCCCAGCTGCCTAAAACCCTTAGGGTCTG NGG 10 8 17 8 24 0 100 5 10
    240604203851_d00y AGGACATCCACATACATAGTGCTCAATTCTTG(+ACTCAC)GTACCAGAAGCAACCTTATTCTACTTACTGA NGG 10 8 17 8 24 0 100 5 10
    240604203849_8etp ATTTGTCAGTAAGTAGAATAAGGTTGCTTCTG(+ACTCAC)GTACCAAGAATTGAGCACTATGTATGTGGAT NGG 10 8 17 8 24 0 100 5 10
    240604203847_b4r3 CTGTCAGGGTTCTCTTTCCAGTGCTTCCTCGG(+ACTCAC)GTACCTGATGGAAGAGAATGTCCTGGAAGCC NGG 10 8 17 8 24 0 100 5 10