Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604203802_b52c TTTGCTAACTTGTTTTGAGTTAGTAAAAGCAG(+ACTCAC)GTACCATGTTCTGCTTTGTTTCCAGTGTGTA NGG 10 8 17 8 24 0 100 5 10
    240604203800_zrb3 TCAATCATTTAATTGACCGCAGTAGTCGCCGG(+ACTCAC)GTACCCGGCACTAAGCATGCACTACATCACA NGG 10 8 17 8 24 0 100 5 10
    240604203758_7mme GCTTCTGTGATGTAGTGCATGCTTAGTGCCGG(+ACTCAC)GTACCCGGCGACTACTGCGGTCAATTAAATG NGG 10 8 17 8 24 0 100 5 10
    240604203757_yut0 TGAGCCACAAAGTTCCCACGAGTTATCATTAG(+ACTCAC)GATCCTGGCCACTGTTTTTGCCATGGTTTTC NGG 10 8 17 8 24 0 100 5 10
    240604203755_d96t GGAAGGAAAACCATGGCAAAAACAGTGGCCAG(+ACTCAC)GATCCTAATGATAACTCGTGGGAACTTTGTG NGG 10 8 17 8 24 0 100 5 10
    240604203753_mf7f AAATCACTGACAATGCCTATATTAGTTAATGG(+ACTCAC)GATCCATCTCAACCCTAAAAAGAAGGAAAAA NGG 10 8 17 8 24 0 100 5 10
    240604203752_9z4z ATTCATTTTTCCTTCTTTTTAGGGTTGAGATG(+ACTCAC)GATCCCATTAACTAATATAGGCATTGTCAGT NGG 10 8 17 8 24 0 100 5 10
    240604203750_rzxc TTGCCTGAACTGATATCTTGGTTTTTCATCCG(+ACTCAC)GATCCAGTCAGGACAGAGACATCAGAAACTA NGG 10 8 17 8 24 0 100 5 10
    240604203749_nkvw TGTATTAGTTTCTGATGTCTCTGTCCTGACTG(+ACTCAC)GATCCGGATGAAAAACCAAGATATCAGTTCA NGG 10 8 17 8 24 0 100 5 10
    240604203747_cu5x AAACCCAAAGCATACGCAGGAGTCAGTTCCTG(+ACTCAC)GTACCGTAGAGTGAGAAAGACACCTGTGCAG NGG 10 8 17 8 24 0 100 5 10