Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604203659_k3o3 CCCGAGGCTGATCAGCCACAACTGGACACCTG(+ACTCAC)GATCCGTCTGACTGGCCCGTGTGTGTGTGTG NGG 10 8 17 8 24 0 100 5 10
    240604203658_mc61 TTAGGGCCCAAGAGACGGCCCAGCAGGTAAAG(+ACTCAC)GTACCTGCTGCCAGGTCTAATGAGCTAAGTT NGG 10 8 17 8 24 0 100 5 10
    240604203656_v0xw AATCAAACTTAGCTCATTAGACCTGGCAGCAG(+ACTCAC)GTACCTTTACCTGCTGGGCCGTCTCTTGGGC NGG 10 8 17 8 24 0 100 5 10
    240604203655_49ka ACATGTGGTTGCCATGGCTTAATGTATGGACG(+ACTCAC)GATCCTCCACTGTCTTGCAATTCATTTGTTG NGG 10 8 17 8 24 0 100 5 10
    240604203653_9fkk TTTTTCAACAAATGAATTGCAAGACAGTGGAG(+ACTCAC)GATCCGTCCATACATTAAGCCATGGCAACCA NGG 10 8 17 8 24 0 100 5 10
    240604203652_0vqa CACCTTTTCTACAGCAATTTTTAGTAATTATG(+ACTCAC)GTACCAAGTTCCCACAAACCTTTGGGTGACT NGG 10 8 17 8 24 0 100 5 10
    240604203650_w9sq TACCCAGTCACCCAAAGGTTTGTGGGAACTTG(+ACTCAC)GTACCATAATTACTAAAAATTGCTGTAGAAA NGG 10 8 17 8 24 0 100 5 10
    240604203648_uviu AGCCTCACCTGGTCCCTGGCCTTCAGCCTCTG(+ACTCAC)GATCCCTGGGTGGGACCCAGAGAATCTCACG NGG 10 8 17 8 24 0 100 5 10
    240604203645_9l5k ATGAGCGTGAGATTCTCTGGGTCCCACCCAGG(+ACTCAC)GATCCAGAGGCTGAAGGCCAGGGACCAGGTG NGG 10 8 17 8 24 0 100 5 10
    240604203644_a2u9 GTTCTTCAGTAGTCTCACACTGGATTGGTCTG(+ACTCAC)GATCCACACGGGTACTCACTTCAGGTCCTGA NGG 10 8 17 8 24 0 100 5 10