Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604203642_e26e TGTATTCAGGACCTGAAGTGAGTACCCGTGTG(+ACTCAC)GATCCAGACCAATCCAGTGTGAGACTACTGA NGG 10 8 17 8 24 0 100 5 10
    240604203641_9j76 ACGGAGCAGCCGGAAACAAGGAGGCCTCGTGG(+ACTCAC)GATCCTTTAGTGCCAAGCCTGGCGTCCCTGG NGG 10 8 17 8 24 0 100 5 10
    240604203639_n5zx CAAGTACAGACCGAGGCCCAGGAGAACGGGCG(+ACTCAC)GTACCGTGTCCAGTCAGCTGCATGGTACTGT NGG 10 8 17 8 24 0 100 5 10
    240604203637_ii6x CGCTGACAGTACCATGCAGCTGACTGGACACG(+ACTCAC)GTACCGCCCGTTCTCCTGGGCCTCGGTCTGT NGG 10 8 17 8 24 0 100 5 10
    240604203636_vqe1 TGAGCTAATTGTGTGTGTGCCTTTCATCTGAG(+ACTCAC)GATCCGAGAGAGCTCCTGGGCAGGTACGAGC NGG 10 8 17 8 24 0 100 5 10
    240604203635_wvqt CGCAGGCTCGTACCTGCCCAGGAGCTCTCTCG(+ACTCAC)GATCCTCAGATGAAAGGCACACACACAATTA NGG 10 8 17 8 24 0 100 5 10
    240604203633_4ddj TACCCTAGTTCTATAGAGCAGCTTGGACACTG(+ACTCAC)GTACCTTGGTCTTCTGCCTTGAAGCTGATTT NGG 10 8 17 8 24 0 100 5 10
    240604203631_l731 CATGGAAATCAGCTTCAAGGCAGAAGACCAAG(+ACTCAC)GTACCAGTGTCCAAGCTGCTCTATAGAACTA NGG 10 8 17 8 24 0 100 5 10
    240604203630_ekyn GTTAGGATTGCAACATGCCTCACTATCTCAAG(+ACTCAC)GTACCTAGATTTTTATTTGCTAAATCTAAAT NGG 10 8 17 8 24 0 100 5 10
    240604203628_36vz TTGATATTTAGATTTAGCAAATAAAAATCTAG(+ACTCAC)GTACCTTGAGATAGTGAGGCATGTTGCAATC NGG 10 8 17 8 24 0 100 5 10