Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604203730_4n2a AGTGCATTAGACAAGGCTCCTAACCACTTTTG(+ACTCAC)GATCCTTTCTGTTTTGCTGCAACCATATTCC NGG 10 8 17 8 24 0 100 5 10
    240604203729_gjri AACAAGAAAATTTGCTTGCTAACTCTTCACTG(+ACTCAC)GTACCAGAATATTTTTAGAGCTAAAATGCCA NGG 10 8 17 8 24 0 100 5 10
    240604203728_iduj CAGCTTGGCATTTTAGCTCTAAAAATATTCTG(+ACTCAC)GTACCAGTGAAGAGTTAGCAAGCAAATTTTC NGG 10 8 17 8 24 0 100 5 10
    240604203727_ydin CTTTCCATCCTGAACCCATACTGCTGGATTTG(+ACTCAC)GATCCATGGACCTGAGGGCCATCAGCCCCAC NGG 10 8 17 8 24 0 100 5 10
    240604203725_x0io CAGGAGTGGGGCTGATGGCCCTCAGGTCCATG(+ACTCAC)GATCCAAATCCAGCAGTATGGGTTCAGGATG NGG 10 8 17 8 24 0 100 5 10
    240604203723_lt9t CAATTTTAATGTTTCCTAAGCATTTTGACCAG(+ACTCAC)GTACCCAGGTTTAAGCTATGAACATTGACAG NGG 10 8 17 8 24 0 100 5 10
    240604203721_b8l2 GGACACTGTCAATGTTCATAGCTTAAACCTGG(+ACTCAC)GTACCTGGTCAAAATGCTTAGGAAACATTAA NGG 10 8 17 8 24 0 100 5 10
    240604203719_4f6l CCTTGAGACCTTTATAAGCAAAAGCAAACCAG(+ACTCAC)GTACCCACCAGTTTGCTCAGACCCAGCAGAC NGG 10 8 17 8 24 0 100 5 10
    240604203716_pxbt ACAAAGTCTGCTGGGTCTGAGCAAACTGGTGG(+ACTCAC)GTACCTGGTTTGCTTTTGCTTATAAAGGTCT NGG 10 8 17 8 24 0 100 5 10
    240604203715_ru45 AAAGTTAAAAATTCCTTAATTTTTTATTCCTG(+ACTCAC)GTACCACTACCACAATTTACAGGGCAATATA NGG 10 8 17 8 24 0 100 5 10