Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604203619_ams9 AGCACTGGGCTCGCTTGGCATGTCCTGGGTTG(+ACTCAC)GATCCCAGTACTCCAACTGAAAAAAAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240604203617_6rzb CTTTTTTTTTTTTTTTTCAGTTGGAGTACTGG(+ACTCAC)GATCCAACCCAGGACATGCCAAGCGAGCCCA NGG 10 8 17 8 24 0 100 5 10
    240604203616_wr24 GCAAGCTGCTTCCCCCGAGCCTTCAGCACAGG(+ACTCAC)GATCCTCTCAACCACAGGCAGCCGGGTTGCC NGG 10 8 17 8 24 0 100 5 10
    240604203614_ifbx TTGCTGGCAACCCGGCTGCCTGTGGTTGAGAG(+ACTCAC)GATCCCTGTGCTGAAGGCTCGGGGGAAGCAG NGG 10 8 17 8 24 0 100 5 10
    240604203613_smf6 AAGGCATAGCAGTGGTACAGGGAACCCAGTGG(+ACTCAC)GTACCCCGCCATCTCTCTTCCAAAGATGAAC NGG 10 8 17 8 24 0 100 5 10
    240604203610_1qvi GGGAGGTTCATCTTTGGAAGAGAGATGGCGGG(+ACTCAC)GTACCCACTGGGTTCCCTGTACCACTGCTAT NGG 10 8 17 8 24 0 100 5 10
    240604203609_l3ws TGTGTCAGCTACTGACAGAGCTGTGCTCTAGG(+ACTCAC)GTACCAGAGGGGCACAAGCATGGCCGATGAT NGG 10 8 17 8 24 0 100 5 10
    240604203607_xrqg CCAGAATCATCGGCCATGCTTGTGCCCCTCTG(+ACTCAC)GTACCCTAGAGCACAGCTCTGTCAGTAGCTG NGG 10 8 17 8 24 0 100 5 10
    240604203605_ao0i AATTATTCAATCACCTGGAAAAAAAAGTCTTG(+ACTCAC)GTACCAATAAAATATGTTTTTCTGTCATCTT NGG 10 8 17 8 24 0 100 5 10
    240604203604_skxv GAAAGAAGATGACAGAAAAACATATTTTATTG(+ACTCAC)GTACCAAGACTTTTTTTTCCAGGTGATTGAA NGG 10 8 17 8 24 0 100 5 10