Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604203246_l6f0 GCTAAAAATTCTGCCTCTCTGTCACTGTGCTG(+ACTCAC)GATCCGTCTAAAGTGGGACAGTTCAATGGTC NGG 10 8 17 8 24 0 100 5 10
    240604203244_hlh7 TTTCAGACCATTGAACTGTCCCACTTTAGACG(+ACTCAC)GATCCAGCACAGTGACAGAGAGGCAGAATTT NGG 10 8 17 8 24 0 100 5 10
    240604203242_jkqa ACCCAGTCTAAGCCCAGAGCTTGTTTGGGAGG(+ACTCAC)GATCCTCTCCTTCCTCCTGCTTGATCCTGTA NGG 10 8 17 8 24 0 100 5 10
    240604203241_vqwp GCTCCTACAGGATCAAGCAGGAGGAAGGAGAG(+ACTCAC)GATCCCTCCCAAACAAGCTCTGGGCTTAGAC NGG 10 8 17 8 24 0 100 5 10
    240604203238_qnfb CTTGATGTGTGAGGGGTAATACCCAGAGTGGG(+ACTCAC)GTACCTGCTTCACAAAGGATAAGGGGAGGGT NGG 10 8 17 8 24 0 100 5 10
    240604203236_zxcd ATTCCACCCTCCCCTTATCCTTTGTGAAGCAG(+ACTCAC)GTACCCCACTCTGGGTATTACCCCTCACACA NGG 10 8 17 8 24 0 100 5 10
    240604203234_lidu TGCCTGCCTGCCTGCCTGCCTGCCTACCTGTG(+ACTCAC)GATCCTGTTCCCCTAAATGGTCTGCCTTGTC NGG 10 8 17 8 24 0 100 5 10
    240604203233_4ygn GGCCAGACAAGGCAGACCATTTAGGGGAACAG(+ACTCAC)GATCCACAGGTAGGCAGGCAGGCAGGCAGGC NGG 10 8 17 8 24 0 100 5 10
    240604203230_zaz3 AGGAAGGCAGGAGGGGTGCCCGTCCCGTTGGG(+ACTCAC)GATCCTAGCACAAGGCCTGACCAGGTCCCTG NGG 10 8 17 8 24 0 100 5 10
    240604203229_m84k AAGTCCAGGGACCTGGTCAGGCCTTGTGCTAG(+ACTCAC)GATCCCCAACGGGACGGGCACCCCTCCTGCC NGG 10 8 17 8 24 0 100 5 10