Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604203302_2cy7 TCTAAGAGTTCTGTGTATATTTCTTCTAGTTG(+ACTCAC)GTACCTACACAAATTAGATTTCTCTTGCTTT NGG 10 8 17 8 24 0 100 5 10
    240604203301_37q8 TGGCAAAAGCAAGAGAAATCTAATTTGTGTAG(+ACTCAC)GTACCAACTAGAAGAAATATACACAGAACTC NGG 10 8 17 8 24 0 100 5 10
    240604203300_67yw GTAAATTAACAACTCAGTTATTAAACTAGCAG(+ACTCAC)GATCCTACAATGATACATCTAGTGAAAGTAG NGG 10 8 17 8 24 0 100 5 10
    240604203258_i5tz TAACTCTACTTTCACTAGATGTATCATTGTAG(+ACTCAC)GATCCTGCTAGTTTAATAACTGAGTTGTTAA NGG 10 8 17 8 24 0 100 5 10
    240604203257_a2o2 ATTAGTTCTGATTCATCCCCGAGGGAAGAGAG(+ACTCAC)GTACCCATGGCTTCAGTGAGCACATGCCAAG NGG 10 8 17 8 24 0 100 5 10
    240604203255_vajk CTGGGCTTGGCATGTGCTCACTGAAGCCATGG(+ACTCAC)GTACCTCTCTTCCCTCGGGGATGAATCAGAA NGG 10 8 17 8 24 0 100 5 10
    240604203253_jkd0 TGGCAAGTGGCCAGCTGAGCCCAGGTTACTTG(+ACTCAC)GTACCCACTCTGCCTTGAGCCTTCCTTCCCC NGG 10 8 17 8 24 0 100 5 10
    240604203252_lb9d CCCTGGGGGAAGGAAGGCTCAAGGCAGAGTGG(+ACTCAC)GTACCAAGTAACCTGGGCTCAGCTGGCCACT NGG 10 8 17 8 24 0 100 5 10
    240604203249_8e4v CCGCTGAGATCCAGCATCCAGCTGTGGCCAGG(+ACTCAC)GATCCACCACCTCTGTGTACACCCTCACCCG NGG 10 8 17 8 24 0 100 5 10
    240604203248_9611 AGCAGCGGGTGAGGGTGTACACAGAGGTGGTG(+ACTCAC)GATCCCTGGCCACAGCTGGATGCTGGATCTC NGG 10 8 17 8 24 0 100 5 10