Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604203317_ms6c CAGACTGGAGTTTTCTGCCTGTTTGTCTCTCG(+ACTCAC)GATCCTCAGAATAGCCGTGTGATGTGAGGTA NGG 10 8 17 8 24 0 100 5 10
    240604203316_jdh3 CTGCCTACCTCACATCACACGGCTATTCTGAG(+ACTCAC)GATCCGAGAGACAAACAGGCAGAAAACTCCA NGG 10 8 17 8 24 0 100 5 10
    240604203314_2dim CTCATGACTTTATATATTCAAAGCTTTTAATG(+ACTCAC)GTACCTGTTACACAGCAAGTACTCTGTGAAT NGG 10 8 17 8 24 0 100 5 10
    240604203313_1sjy TCAGCATTCACAGAGTACTTGCTGTGTAACAG(+ACTCAC)GTACCATTAAAAGCTTTGAATATATAAAGTC NGG 10 8 17 8 24 0 100 5 10
    240604203311_hjop TAACCAATTGCTTAGGGGCAGGAAGATGTGGG(+ACTCAC)GATCCCACCTTGCCAGAGCTCTTACCATCTT NGG 10 8 17 8 24 0 100 5 10
    240604203309_2fw8 TGGCAAAGATGGTAAGAGCTCTGGCAAGGTGG(+ACTCAC)GATCCCCACATCTTCCTGCCCCTAAGCAATT NGG 10 8 17 8 24 0 100 5 10
    240604203308_tou2 TAGGCTCACACACTAAGATTGACTGGCATATG(+ACTCAC)GATCCCTTTCTTAGATGGTGGTGTATAGCTT NGG 10 8 17 8 24 0 100 5 10
    240604203306_umwq TCTTGAAGCTATACACCACCATCTAAGAAAGG(+ACTCAC)GATCCATATGCCAGTCAATCTTAGTGTGTGA NGG 10 8 17 8 24 0 100 5 10
    240604203305_u3c6 ATTTTAAATTTTTTACCAAGCACATTCTGAAG(+ACTCAC)GTACCAACAAATGACATTATCTACTTCCTAC NGG 10 8 17 8 24 0 100 5 10
    240604203304_881c TACTAGTAGGAAGTAGATAATGTCATTTGTTG(+ACTCAC)GTACCTTCAGAATGTGCTTGGTAAAAAATTT NGG 10 8 17 8 24 0 100 5 10