Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604203330_4czg TAAGGATAAATGCATTAAGTATAACTAATTTG(+ACTCAC)GTACCATACAAGTATTTTCTTTGGAATTTTG NGG 10 8 17 8 24 0 100 5 10
    240604203329_0o95 ACATACAAAATTCCAAAGAAAATACTTGTATG(+ACTCAC)GTACCAAATTAGTTATACTTAATGCATTTAT NGG 10 8 17 8 24 0 100 5 10
    240604203328_m4o3 ATGCCCCTTTGTAATTTTTCTTGCCTTCTGTG(+ACTCAC)GTACCTGCTCTCTAAAGCAGTAGTTTCTCCA NGG 10 8 17 8 24 0 100 5 10
    240604203326_e6ge AAAGCTGGAGAAACTACTGCTTTAGAGAGCAG(+ACTCAC)GTACCACAGAAGGCAAGAAAAATTACAAAGG NGG 10 8 17 8 24 0 100 5 10
    240604203325_41sr TGTCAGTTTTTCTCTTTAGTGAAATGATACAG(+ACTCAC)GTACCAGCAAAGTGACAATATGTCCTTTGAT NGG 10 8 17 8 24 0 100 5 10
    240604203324_fza3 TGGACATCAAAGGACATATTGTCACTTTGCTG(+ACTCAC)GTACCTGTATCATTTCACTAAAGAGAAAAAC NGG 10 8 17 8 24 0 100 5 10
    240604203322_g5bu AGTCCTCTAGTCTTGGCTGTGGGGGCTTCCAG(+ACTCAC)GTACCACTTGGGGAGAGGACTCAAGTAAGAC NGG 10 8 17 8 24 0 100 5 10
    240604203321_mqax CACTTGTCTTACTTGAGTCCTCTCCCCAAGTG(+ACTCAC)GTACCTGGAAGCCCCCACAGCCAAGACTAGA NGG 10 8 17 8 24 0 100 5 10
    240604203320_e1ij GGGTTGGGAACAAGGGGGTGGGGTCATCAGAG(+ACTCAC)GTACCTGTTACTTTAGGGACCCTCTCTGTCT NGG 10 8 17 8 24 0 100 5 10
    240604203318_kixh CTCTCAGACAGAGAGGGTCCCTAAAGTAACAG(+ACTCAC)GTACCTCTGATGACCCCACCCCCTTGTTCCC NGG 10 8 17 8 24 0 100 5 10