Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604203434_ry9o ATAAACTAGAAAACATAGATGCACAGGACTCG(+ACTCAC)GATCCATGATATTTACACTGGGAAATGTTCT NGG 10 8 17 8 24 0 100 5 10
    240604203433_g0rt CATTTAGAACATTTCCCAGTGTAAATATCATG(+ACTCAC)GATCCGAGTCCTGTGCATCTATGTTTTCTAG NGG 10 8 17 8 24 0 100 5 10
    240604203431_u7sp ACGATAAGCAATGGTCAGACTTTCCTTCTGTG(+ACTCAC)GATCCTTATGCCAATTCAGCTACTAATCGGT NGG 10 8 17 8 24 0 100 5 10
    240604203430_t0sp GAATGACCGATTAGTAGCTGAATTGGCATAAG(+ACTCAC)GATCCACAGAAGGAAAGTCTGACCATTGCTT NGG 10 8 17 8 24 0 100 5 10
    240604203428_8pxb AATATGAGGGAGGCACATTTTCTAGTGGCTGG(+ACTCAC)GATCCTCTTCCAAACCATAAGAAAAAAGGCA NGG 10 8 17 8 24 0 100 5 10
    240604203427_zytg ACCACTGCCTTTTTTCTTATGGTTTGGAAGAG(+ACTCAC)GATCCCAGCCACTAGAAAATGTGCCTCCCTC NGG 10 8 17 8 24 0 100 5 10
    240604203425_aw1p ACAGGGTTAAAGTTTTGGTCCTCAGCCCATTG(+ACTCAC)GTACCATTAGGAAACAGGGTCTAGTCAGAGA NGG 10 8 17 8 24 0 100 5 10
    240604203424_2sck GAAATTCTCTGACTAGACCCTGTTTCCTAATG(+ACTCAC)GTACCAATGGGCTGAGGACCAAAACTTTAAC NGG 10 8 17 8 24 0 100 5 10
    240604203422_aca1 CGGACTAGGTGGTTAGTGGATACTATTGTTTG(+ACTCAC)GATCCTGACTGTTACAGGGTTAAAGTTTTGG NGG 10 8 17 8 24 0 100 5 10
    240604203421_3mya GAGGACCAAAACTTTAACCCTGTAACAGTCAG(+ACTCAC)GATCCAAACAATAGTATCCACTAACCACCTA NGG 10 8 17 8 24 0 100 5 10