Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604203344_r4li TGAGGCACAGTCTCTGTTCTGGAATTCACAAG(+ACTCAC)GTACCTGTAATAAACATCCAAACAAGAGTCA NGG 10 8 17 8 24 0 100 5 10
    240604203343_483j ATTTGTGACTCTTGTTTGGATGTTTATTACAG(+ACTCAC)GTACCTTGTGAATTCCAGAACAGAGACTGTG NGG 10 8 17 8 24 0 100 5 10
    240604203342_i91t TCTAATGTAATAATGTTTTTTAAAGCACTCTG(+ACTCAC)GTACCTACATGCCATCAATTCCACTTTCTTC NGG 10 8 17 8 24 0 100 5 10
    240604203340_a7n6 CTTACGAAGAAAGTGGAATTGATGGCATGTAG(+ACTCAC)GTACCAGAGTGCTTTAAAAAACATTATTACA NGG 10 8 17 8 24 0 100 5 10
    240604203339_afe0 ATATAATTTTAGTTTAGCAATAATTTTGACTG(+ACTCAC)GTACCAATAACTGTTTTTTAAAATTCCATAC NGG 10 8 17 8 24 0 100 5 10
    240604203338_6sck CATAAGTATGGAATTTTAAAAAACAGTTATTG(+ACTCAC)GTACCAGTCAAAATTATTGCTAAACTAAAAT NGG 10 8 17 8 24 0 100 5 10
    240604203336_fem0 TAATAACAGACCAGCGATTTTATAGAGGCCAG(+ACTCAC)GATCCTGAACTGGGGCATCCTCTCTGGAGTG NGG 10 8 17 8 24 0 100 5 10
    240604203334_f7vt GCCTGCACTCCAGAGAGGATGCCCCAGTTCAG(+ACTCAC)GATCCTGGCCTCTATAAAATCGCTGGTCTGT NGG 10 8 17 8 24 0 100 5 10
    240604203333_1rcp TTGGACTTTGTAAGCCTGAAAAAGAATATTTG(+ACTCAC)GATCCCTCTACTAAACAAGGGCTATGTATTA NGG 10 8 17 8 24 0 100 5 10
    240604203332_fft6 CTGTATAATACATAGCCCTTGTTTAGTAGAGG(+ACTCAC)GATCCAAATATTCTTTTTCAGGCTTACAAAG NGG 10 8 17 8 24 0 100 5 10