Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604203449_4on2 GGGGGGGCACGGAGGAAAGCAAGGCTGTGTGG(+ACTCAC)GATCCACTGGAGCCTCAGGAGGAAGGGAACA NGG 10 8 17 8 24 0 100 5 10
    240604203447_fux1 CCCAGTGTTCCCTTCCTCCTGAGGCTCCAGTG(+ACTCAC)GATCCCACACAGCCTTGCTTTCCTCCGTGCC NGG 10 8 17 8 24 0 100 5 10
    240604203446_2f9d GTATTGGTGCAGAGGAGAGCTCTTGGTGCTGG(+ACTCAC)GATCCTATGCCACTCCCCACTCCATCTCTAA NGG 10 8 17 8 24 0 100 5 10
    240604203444_yc5y TCTGTTTAGAGATGGAGTGGGGAGTGGCATAG(+ACTCAC)GATCCCAGCACCAAGAGCTCTCCTCTGCACC NGG 10 8 17 8 24 0 100 5 10
    240604203443_qev4 AAGTATAAACCTCGATTTTCCTGGGCAGACTG(+ACTCAC)GATCCTATGATCAACCTAGGAATAGGGATTA NGG 10 8 17 8 24 0 100 5 10
    240604203442_wqvj CAGTTTAATCCCTATTCCTAGGTTGATCATAG(+ACTCAC)GATCCAGTCTGCCCAGGAAAATCGAGGTTTA NGG 10 8 17 8 24 0 100 5 10
    240604203440_b5ns TTTTATATCAGTGTGTTGAATTTTGACTTTAG(+ACTCAC)GTACCTGGAAATGTAATATCCTTGCTATAAA NGG 10 8 17 8 24 0 100 5 10
    240604203439_i9nb CATGTTTTATAGCAAGGATATTACATTTCCAG(+ACTCAC)GTACCTAAAGTCAAAATTCAACACACTGATA NGG 10 8 17 8 24 0 100 5 10
    240604203437_3dm1 AGTCCTTACAAGGATGGAGTGCGTGCAAAATG(+ACTCAC)GTACCTTACTGTTTCTAGAACAGCCTTCTGC NGG 10 8 17 8 24 0 100 5 10
    240604203436_7v8h GAAGAGCAGAAGGCTGTTCTAGAAACAGTAAG(+ACTCAC)GTACCATTTTGCACGCACTCCATCCTTGTAA NGG 10 8 17 8 24 0 100 5 10