Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604203520_q34j TAATTTTTTGTAACTTTCCACTACTCTACCTG(+ACTCAC)GTACCATATCTGTGGATTTTGGCCAATATTA NGG 10 8 17 8 24 0 100 5 10
    240604203519_cdq2 AGTTTTAATATTGGCCAAAATCCACAGATATG(+ACTCAC)GTACCAGGTAGAGTAGTGGAAAGTTACAAAA NGG 10 8 17 8 24 0 100 5 10
    240604203517_qewu TGTGGCAGGGTGGATAATGCGTGAGGACTTGG(+ACTCAC)GATCCTGATGGACTTTTAATTGTACCCACAA NGG 10 8 17 8 24 0 100 5 10
    240604203516_zabw TTGAGTTGTGGGTACAATTAAAAGTCCATCAG(+ACTCAC)GATCCCAAGTCCTCACGCATTATCCACCCTG NGG 10 8 17 8 24 0 100 5 10
    240604203515_thfi AATCCTCTCTACTTGACTCAGGGGCTCACAAG(+ACTCAC)GTACCATTATATGCAAAAGTACTGTATATTT NGG 10 8 17 8 24 0 100 5 10
    240604203514_gz3w AGGAAAAATATACAGTACTTTTGCATATAATG(+ACTCAC)GTACCTTGTGAGCCCCTGAGTCAAGTAGAGA NGG 10 8 17 8 24 0 100 5 10
    240604203511_kk61 CTAAATTTAAAAGTCTTAATGAATCTCCTTTG(+ACTCAC)GATCCCTGAATGGTCCAAGATCTTGGAGTCT NGG 10 8 17 8 24 0 100 5 10
    240604203509_ae3a GCTCCAGACTCCAAGATCTTGGACCATTCAGG(+ACTCAC)GATCCAAAGGAGATTCATTAAGACTTTTAAA NGG 10 8 17 8 24 0 100 5 10
    240604203508_d4i8 AAAGTATGAATGTGTTCTGCACAGCAAACTGG(+ACTCAC)GATCCTTATCAGTACAACCTGAAGACTAGAG NGG 10 8 17 8 24 0 100 5 10
    240604203506_6nyl AGGGACTCTAGTCTTCAGGTTGTACTGATAAG(+ACTCAC)GATCCCAGTTTGCTGTGCAGAACACATTCAT NGG 10 8 17 8 24 0 100 5 10