Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604202947_ta7v GAAACAAAACCAAAACCCAACTTCTCAGTAGG(+ACTCAC)GTACCACTAGCTGGCCTGTGGACAACAGTCT NGG 10 8 17 8 24 0 100 5 10
    240604202946_fbj9 TGAGGAGACTGTTGTCCACAGGCCAGCTAGTG(+ACTCAC)GTACCCTACTGAGAAGTTGGGTTTTGGTTTT NGG 10 8 17 8 24 0 100 5 10
    240604202943_9sj6 CTATCTGGTCTGCCCCTGTGTAGCTCCCATGG(+ACTCAC)GATCCCTTAAAATGCATCCTTTTTAATTTTC NGG 10 8 17 8 24 0 100 5 10
    240604202941_nf5d GCCCGGAAAATTAAAAAGGATGCATTTTAAGG(+ACTCAC)GATCCCATGGGAGCTACACAGGGGCAGACCA NGG 10 8 17 8 24 0 100 5 10
    240604202938_zlxm ATGCGTCCTTGCCCACCTCTCACCAAGCCCCG(+ACTCAC)GATCCTTGCGGACTTCCCCAGGTGCCCCCTG NGG 10 8 17 8 24 0 100 5 10
    240604202937_2p9n GGGGACAGGGGGCACCTGGGGAAGTCCGCAAG(+ACTCAC)GATCCGGGGCTTGGTGAGAGGTGGGCAAGGA NGG 10 8 17 8 24 0 100 5 10
    240604202936_xnm2 ACCCGCAGCTCCTAGTCTCTAATGCCTGGTAG(+ACTCAC)GATCCCATGGGCCTTTGCGACGCCACGTTAG NGG 10 8 17 8 24 0 100 5 10
    240604202933_np51 GTTGCCTAACGTGGCGTCGCAAAGGCCCATGG(+ACTCAC)GATCCTACCAGGCATTAGAGACTAGGAGCTG NGG 10 8 17 8 24 0 100 5 10
    240604202932_3pt9 GGACATCAATCTAAAGCCTCGAGGCCATGTTG(+ACTCAC)GATCCACCCTGACCCGGTCCTGTAGCAACTA NGG 10 8 17 8 24 0 100 5 10
    240604202930_xaz6 TTTTTTAGTTGCTACAGGACCGGGTCAGGGTG(+ACTCAC)GATCCAACATGGCCTCGAGGCTTTAGATTGA NGG 10 8 17 8 24 0 100 5 10