Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604202839_6tpt TAGCTAGCTGGATGCTGTTTACTGGGTGAAAG(+ACTCAC)GTACCTTGGGAGTTTTCATGGCTCCAAGCAG NGG 10 8 17 8 24 0 100 5 10
    240604202837_vkn8 CATCCCTGCTTGGAGCCATGAAAACTCCCAAG(+ACTCAC)GTACCTTTCACCCAGTAAACAGCATCCAGCT NGG 10 8 17 8 24 0 100 5 10
    240604202835_b0nc TGGCCCTACAGAACACACCTGAGCCTCTTGGG(+ACTCAC)GTACCATCGCAGGGTAGCCCTCCCTAGGGCC NGG 10 8 17 8 24 0 100 5 10
    240604202834_epgi AACTAGGCCCTAGGGAGGGCTACCCTGCGATG(+ACTCAC)GTACCCCAAGAGGCTCAGGTGTGTTCTGTAG NGG 10 8 17 8 24 0 100 5 10
    240604202832_kmb9 CCAAGAACCAACTAGCTGCCTCAGGTCTCGTG(+ACTCAC)GATCCCAGATGGACATGGCAGTAACTCAGAG NGG 10 8 17 8 24 0 100 5 10
    240604202830_kyhb ACCGGCTCTGAGTTACTGCCATGTCCATCTGG(+ACTCAC)GATCCACGAGACCTGAGGCAGCTAGTTGGTT NGG 10 8 17 8 24 0 100 5 10
    240604202828_vxn0 TCTCACCTGAGAAATGGTGCAAACCAACCAGG(+ACTCAC)GATCCTTAGGCTACTGAGAAGGCTGTCTGCT NGG 10 8 17 8 24 0 100 5 10
    240604202826_bgp8 AGGTAAGCAGACAGCCTTCTCAGTAGCCTAAG(+ACTCAC)GATCCCTGGTTGGTTTGCACCATTTCTCAGG NGG 10 8 17 8 24 0 100 5 10
    240604202823_nhhg GGGCCCTGTGACCTTCAACATCTGGCAGGGGG(+ACTCAC)GATCCTGGGACAGGGGGTGGAGAAGGCAGTT NGG 10 8 17 8 24 0 100 5 10
    240604202821_7y54 GTGTCAACTGCCTTCTCCACCCCCTGTCCCAG(+ACTCAC)GATCCCCCCTGCCAGATGTTGAAGGTCACAG NGG 10 8 17 8 24 0 100 5 10