Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604202803_sg9y AAATTGCTGAGTGAAGCCATGCAAGACGGAGG(+ACTCAC)GATCCAGGATAGGGTCCAGTCCCTGTCCTGT NGG 10 8 17 8 24 0 100 5 10
    240604202801_5cgi AGCAGACAGGACAGGGACTGGACCCTATCCTG(+ACTCAC)GATCCCTCCGTCTTGCATGGCTTCACTCAGC NGG 10 8 17 8 24 0 100 5 10
    240604202759_mr2a GGGGGTGAGGAAAGAGAGAAGGGGAGTCGGAG(+ACTCAC)GATCCAGCAGGCCACTCTTTCCACCTCCCAT NGG 10 8 17 8 24 0 100 5 10
    240604202757_f37n GTCCCATGGGAGGTGGAAAGAGTGGCCTGCTG(+ACTCAC)GATCCTCCGACTCCCCTTCTCTCTTTCCTCA NGG 10 8 17 8 24 0 100 5 10
    240604202756_73vb TGCAGTGGCCACAAAGGCCAGAGAGGCTGTTG(+ACTCAC)GATCCCCTTGAGCTGGAGTTACAGGCGATCG NGG 10 8 17 8 24 0 100 5 10
    240604202754_wizw GCAAACGATCGCCTGTAACTCCAGCTCAAGGG(+ACTCAC)GATCCAACAGCCTCTCTGGCCTTTGTGGCCA NGG 10 8 17 8 24 0 100 5 10
    240604202750_342g CATGGCTTGGGTTGTGGACCTTCTTCCCCATG(+ACTCAC)GATCCAGAGTGGCACAGGGTCCTGCCCTGAG NGG 10 8 17 8 24 0 100 5 10
    240604202749_njzm CTGTGCTCAGGGCAGGACCCTGTGCCACTCTG(+ACTCAC)GATCCATGGGGAAGAAGGTCCACAACCCAAG NGG 10 8 17 8 24 0 100 5 10
    240604202748_g655 TGACCTGGCCAATGTACAGGCCTTTGTCTCAG(+ACTCAC)GATCCTTGCCTGGTTTGCACAATAGGAAATG NGG 10 8 17 8 24 0 100 5 10
    240604202747_gmpu CACCACATTTCCTATTGTGCAAACCAGGCAAG(+ACTCAC)GATCCTGAGACAAAGGCCTGTACATTGGCCA NGG 10 8 17 8 24 0 100 5 10