Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604202744_vfpc CAACACTGCACCACTGAACTGTGTCCCCCAGG(+ACTCAC)GTACCATCACAGAGAATGGACAAAAAATGAG NGG 10 8 17 8 24 0 100 5 10
    240604202743_n0ne GTGGGCTCATTTTTTGTCCATTCTCTGTGATG(+ACTCAC)GTACCCTGGGGGACACAGTTCAGTGGTGCAG NGG 10 8 17 8 24 0 100 5 10
    240604202741_lidn TGCTAGTTGCTCTGAAAGATTGGGACCTCTTG(+ACTCAC)GATCCCCCCCCCCATGGACTCTGATTGTCTG NGG 10 8 17 8 24 0 100 5 10
    240604202739_h12d ATAGACAGACAATCAGAGTCCATGGGGGGGGG(+ACTCAC)GATCCAAGAGGTCCCAATCTTTCAGAGCAAC NGG 10 8 17 8 24 0 100 5 10
    240604202737_z14s AACCCCCGCCAGCCCAGTAGGGTAGCCAGGAG(+ACTCAC)GATCCCTGAGTCCAACCCGTAAACACTAGAG NGG 10 8 17 8 24 0 100 5 10
    240604202735_tzbn TGTGGCTCTAGTGTTTACGGGTTGGACTCAGG(+ACTCAC)GATCCTCCTGGCTACCCTACTGGGCTGGCGG NGG 10 8 17 8 24 0 100 5 10
    240604202734_qg3r GCTATAGAGGGCAAACCTGTTTGTATTCATGG(+ACTCAC)GATCCAACAAAATACATGAAAGGATGAGCCA NGG 10 8 17 8 24 0 100 5 10
    240604202732_9e13 CATATTGGCTCATCCTTTCATGTATTTTGTTG(+ACTCAC)GATCCCATGAATACAAACAGGTTTGCCCTCT NGG 10 8 17 8 24 0 100 5 10
    240604202731_38lq TCACTGAGCTCTGTTCTCTGTCTGCAGCACAG(+ACTCAC)GTACCAAAGAGGGGTGCAATGGAGGGCTAGC NGG 10 8 17 8 24 0 100 5 10
    240604202729_zp97 TCAGAGCTAGCCCTCCATTGCACCCCTCTTTG(+ACTCAC)GTACCTGTGCTGCAGACAGAGAACAGAGCTC NGG 10 8 17 8 24 0 100 5 10