Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604202728_4aij AGTCATAGCTTCTCAGCAGTTGTGAAAAGTGG(+ACTCAC)GTACCATAGCCAGCTCCACTAGCTTCAGGGC NGG 10 8 17 8 24 0 100 5 10
    240604202726_ml3j CCAATGCCCTGAAGCTAGTGGAGCTGGCTATG(+ACTCAC)GTACCCACTTTTCACAACTGCTGAGAAGCTA NGG 10 8 17 8 24 0 100 5 10
    240604202725_el6r TTTGCTGGACCTGATCCCCAAGCACAGGAGTG(+ACTCAC)GTACCCTCCACAGAAGCTGGGGGAGAGTCTG NGG 10 8 17 8 24 0 100 5 10
    240604202723_eftd TGCTGCAGACTCTCCCCCAGCTTCTGTGGAGG(+ACTCAC)GTACCACTCCTGTGCTTGGGGATCAGGTCCA NGG 10 8 17 8 24 0 100 5 10
    240604202721_zw6v ACTATTAACTATAATAGAAGTCACTAGGAAGG(+ACTCAC)GTACCATGCTCTCAGAATCCCCACGTCCCAG NGG 10 8 17 8 24 0 100 5 10
    240604202720_hlrc CGTGGCTGGGACGTGGGGATTCTGAGAGCATG(+ACTCAC)GTACCCTTCCTAGTGACTTCTATTATAGTTA NGG 10 8 17 8 24 0 100 5 10
    240604202718_yuuv TGGTCCTTGGCACAGCCCCTCCCTCATGGCTG(+ACTCAC)GATCCTCAGATCATAATAGGGCCTTGGAGCA NGG 10 8 17 8 24 0 100 5 10
    240604202717_w5ik AACCCTGCTCCAAGGCCCTATTATGATCTGAG(+ACTCAC)GATCCAGCCATGAGGGAGGGGCTGTGCCAAG NGG 10 8 17 8 24 0 100 5 10
    240604202715_ew0m TTCACCTTCCGGAGAAAACATTTCAAGCGGAG(+ACTCAC)GATCCGGTATCTCGGGATTCCGAAGTCTCCC NGG 10 8 17 8 24 0 100 5 10
    240604202713_99cw ACACCGGGAGACTTCGGAATCCCGAGATACCG(+ACTCAC)GATCCTCCGCTTGAAATGTTTTCTCCGGAAG NGG 10 8 17 8 24 0 100 5 10