Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604202711_ntxw ATTGCACTTATTTCTATGAACCCCATGGAGGG(+ACTCAC)GATCCCCAATGCTGCTGCCTCCAGGCGAGGC NGG 10 8 17 8 24 0 100 5 10
    240604202709_gzbc GTCATGCCTCGCCTGGAGGCAGCAGCATTGGG(+ACTCAC)GATCCCCTCCATGGGGTTCATAGAAATAAGT NGG 10 8 17 8 24 0 100 5 10
    240604202708_akw4 GGTACTTCCTGCAGAGCCTGACACCAGAGTTG(+ACTCAC)GATCCCTGGGACCCACATGATGAGAGATCCA NGG 10 8 17 8 24 0 100 5 10
    240604202705_uj9i GAAGCTGGATCTCTCATCATGTGGGTCCCAGG(+ACTCAC)GATCCAACTCTGGTGTCAGGCTCTGCAGGAA NGG 10 8 17 8 24 0 100 5 10
    240604202703_aenz CAGATTGATGTTCAACATGGGGTCTGAGTAGG(+ACTCAC)GATCCCAACTAAAAAGCCATAACCTTCAGAA NGG 10 8 17 8 24 0 100 5 10
    240604202701_569s TTCAGTTCTGAAGGTTATGGCTTTTTAGTTGG(+ACTCAC)GATCCCTACTCAGACCCCATGTTGAACATCA NGG 10 8 17 8 24 0 100 5 10
    240604202700_676g TGTTGCTGTGAGGTCTTATGGCAGCTGCTCTG(+ACTCAC)GATCCATTGCTGGCTTCTTTGGCACCTCCAG NGG 10 8 17 8 24 0 100 5 10
    240604202659_aee2 TGAACCTGGAGGTGCCAAAGAAGCCAGCAATG(+ACTCAC)GATCCAGAGCAGCTGCCATAAGACCTCACAG NGG 10 8 17 8 24 0 100 5 10
    240604202657_0eoh GGTTTGTCCAAATGTGTGGCATGGGAGATAGG(+ACTCAC)GTACCCCTGAAAGCAAGGAAAGGAGCCCAGC NGG 10 8 17 8 24 0 100 5 10
    240604202655_w61c CCACAGCTGGGCTCCTTTCCTTGCTTTCAGGG(+ACTCAC)GTACCCTATCTCCCATGCCACACATTTGGAC NGG 10 8 17 8 24 0 100 5 10