Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604202621_0pzo TCGTAGTCGCTAAAATTCACTCAATTCACATG(+ACTCAC)GATCCCAGCTGTGTGTTGTAAGGCTGAGTCA NGG 10 8 17 8 24 0 100 5 10
    240604202619_ydc3 TCCACTGACTCAGCCTTACAACACACAGCTGG(+ACTCAC)GATCCATGTGAATTGAGTGAATTTTAGCGAC NGG 10 8 17 8 24 0 100 5 10
    240604202618_jirr TCCACTTTGAACGTTTTCAGAAATCCCATAAG(+ACTCAC)GATCCTGGAGTGATAGATGATTTCTGTCTTG NGG 10 8 17 8 24 0 100 5 10
    240604202616_tfw3 TTCACCAAGACAGAAATCATCTATCACTCCAG(+ACTCAC)GATCCTTATGGGATTTCTGAAAACGTTCAAA NGG 10 8 17 8 24 0 100 5 10
    240604202615_hgn2 TGTGTATGTCTGTGTACCAGAAGACGACATTG(+ACTCAC)GATCCCATAGATCTGTTGTATGGATGGCTGT NGG 10 8 17 8 24 0 100 5 10
    240604202613_eel4 GACTTACAGCCATCCATACAACAGATCTATGG(+ACTCAC)GATCCAATGTCGTCTTCTGGTACACAGACAT NGG 10 8 17 8 24 0 100 5 10
    240604202611_poxs GTAAAGTAGTAGCTAACCCCTCCAAGGCCCAG(+ACTCAC)GATCCTAGTTCCAGTGTTACAGAAATGTTTC NGG 10 8 17 8 24 0 100 5 10
    240604202610_fsap AAAAGGAAACATTTCTGTAACACTGGAACTAG(+ACTCAC)GATCCTGGGCCTTGGAGGGGTTAGCTACTAC NGG 10 8 17 8 24 0 100 5 10
    240604202608_32m1 CACTTTACTGTTTCAATGGTAATAATGCACCG(+ACTCAC)GTACCTCAATTCAACTGCTACAAATAAATGT NGG 10 8 17 8 24 0 100 5 10
    240604202606_irdo TTTTGACATTTATTTGTAGCAGTTGAATTGAG(+ACTCAC)GTACCGGTGCATTATTACCATTGAAACAGTA NGG 10 8 17 8 24 0 100 5 10