Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604202548_v0bc ATTGCAAGCAAGGGATTAAAGATTTGTCTTGG(+ACTCAC)GTACCAAATCACATAACGAGGATTCAAAGGA NGG 10 8 17 8 24 0 100 5 10
    240604202546_kn87 GTTAATCCTTTGAATCCTCGTTATGTGATTTG(+ACTCAC)GTACCCAAGACAAATCTTTAATCCCTTGCTT NGG 10 8 17 8 24 0 100 5 10
    240604202545_ovk1 TGGATTCGTATGGCTAACGTCAGTGAATCAAG(+ACTCAC)GATCCCAGGGGATGCCCATTGCTGGGCATCT NGG 10 8 17 8 24 0 100 5 10
    240604202542_jfg8 AACTGAGATGCCCAGCAATGGGCATCCCCTGG(+ACTCAC)GATCCTTGATTCACTGACGTTAGCCATACGA NGG 10 8 17 8 24 0 100 5 10
    240604202540_wij0 ATTGCAAGCAAGGGATTAAAGATTTGTCTTGG(+ACTCAC)GTACCAAATCACATAACGAGGATTCAAAGGA NGG 10 8 17 8 24 0 100 5 10
    240604202539_1zjz GTTAATCCTTTGAATCCTCGTTATGTGATTTG(+ACTCAC)GTACCCAAGACAAATCTTTAATCCCTTGCTT NGG 10 8 17 8 24 0 100 5 10
    240604202538_6oud TCTACATTTTAATTTTTATGATTCCTGATGTG(+ACTCAC)GATCCATTACAAACTTCTCAGAAGTTTGGAG NGG 10 8 17 8 24 0 100 5 10
    240604202536_muds ATGCACTCCAAACTTCTGAGAAGTTTGTAATG(+ACTCAC)GATCCACATCAGGAATCATAAAAATTAAAAT NGG 10 8 17 8 24 0 100 5 10
    240604202534_gn4g GGAAGAAAGGTGGTGTCCTTCCGGACTCCGGG(+ACTCAC)GATCCAGGCCTGAATATGGGAGGCACCCAGG NGG 10 8 17 8 24 0 100 5 10
    240604202532_qyj5 CTGAGCCTGGGTGCCTCCCATATTCAGGCCTG(+ACTCAC)GATCCCCGGAGTCCGGAAGGACACCACCTTT NGG 10 8 17 8 24 0 100 5 10