Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604202458_5l3k CAGAGCCAACAGCTTCTCATTCTAAAGCAAGG(+ACTCAC)GATCCCTAGAGTTTGGAGCTTCATGTTGGGG NGG 10 8 17 8 24 0 100 5 10
    240604202456_u0i9 CCTAACCCCAACATGAAGCTCCAAACTCTAGG(+ACTCAC)GATCCCTTGCTTTAGAATGAGAAGCTGTTGG NGG 10 8 17 8 24 0 100 5 10
    240604202455_eh1o GCCTCTGTGTAGTCTGGATCTACTGACACTGG(+ACTCAC)GTACCAGGGTTTCCTTCTGTTGACTGTGATT NGG 10 8 17 8 24 0 100 5 10
    240604202452_lleg GCTGCAATCACAGTCAACAGAAGGAAACCCTG(+ACTCAC)GTACCCAGTGTCAGTAGATCCAGACTACACA NGG 10 8 17 8 24 0 100 5 10
    240604202451_biax GTGGAATTTCTCCAGGGTGGAATTTTAGACTG(+ACTCAC)GTACCAGAAGTCGGCAAGGCGTGACTGCTGG NGG 10 8 17 8 24 0 100 5 10
    240604202450_1yxf TGTGTCCAGCAGTCACGCCTTGCCGACTTCTG(+ACTCAC)GTACCAGTCTAAAATTCCACCCTGGAGAAAT NGG 10 8 17 8 24 0 100 5 10
    240604202448_8k0o CTACCACTAAGCTATACTTTCGAACTTTTTAG(+ACTCAC)GATCCTTTGTGCTGGAGGAGAGCAAAGCAGG NGG 10 8 17 8 24 0 100 5 10
    240604202447_ua3r AAGGCCCTGCTTTGCTCTCCTCCAGCACAAAG(+ACTCAC)GATCCTAAAAAGTTCGAAAGTATAGCTTAGT NGG 10 8 17 8 24 0 100 5 10
    240604202445_6rm9 ATCCAGGTTCTATGGCCAGCTGGCCACGAAGG(+ACTCAC)GTACCTTGGTCCTAGGCAACCCACCTCTCGG NGG 10 8 17 8 24 0 100 5 10
    240604202443_6q3j GAGAGCCGAGAGGTGGGTTGCCTAGGACCAAG(+ACTCAC)GTACCCTTCGTGGCCAGCTGGCCATAGAACC NGG 10 8 17 8 24 0 100 5 10