Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604202426_wxj6 TCCAGGGAATTAGCCTCCTCTGGTGACTATGG(+ACTCAC)GTACCAGGTACACATAAGGTGCACAAACATA NGG 10 8 17 8 24 0 100 5 10
    240604202424_yyya TCATGTATGTTTGTGCACCTTATGTGTACCTG(+ACTCAC)GTACCCATAGTCACCAGAGGAGGCTAATTCC NGG 10 8 17 8 24 0 100 5 10
    240604202423_yzhb CTTCAGTGAAGCATGCCAGAGGCAGTGAACGG(+ACTCAC)GTACCATAAGTGGCTGCTGGGATATACCAGG NGG 10 8 17 8 24 0 100 5 10
    240604202421_5wi2 GCCTACCTGGTATATCCCAGCAGCCACTTATG(+ACTCAC)GTACCCGTTCACTGCCTCTGGCATGCTTCAC NGG 10 8 17 8 24 0 100 5 10
    240604202418_5lvn GAGGGTTTTCAGTTGTGACAGCCCCACCCCCG(+ACTCAC)GTACCATCTCTCTCCTCAATGTAGTCATGCA NGG 10 8 17 8 24 0 100 5 10
    240604202417_icya GTGCTTGCATGACTACATTGAGGAGAGAGATG(+ACTCAC)GTACCGGGGGTGGGGCTGTCACAACTGAAAA NGG 10 8 17 8 24 0 100 5 10
    240604202415_9ju1 CTGGGGCCAATAAGCTGGCTTAGTAGGTAAAG(+ACTCAC)GTACCTGCTGCCAAGCCTGATGCCCTGAGTT NGG 10 8 17 8 24 0 100 5 10
    240604202414_8sgt GATTGAACTCAGGGCATCAGGCTTGGCAGCAG(+ACTCAC)GTACCTTTACCTACTAAGCCAGCTTATTGGC NGG 10 8 17 8 24 0 100 5 10
    240604202413_0kb4 TTGCCTGCGTTTATGTCTGTGTGAGAGTTTTG(+ACTCAC)GATCCCCTAAAACGAGTTGCAAGCAGGTGTG NGG 10 8 17 8 24 0 100 5 10
    240604202411_2j6n TAACTCACACCTGCTTGCAACTCGTTTTAGGG(+ACTCAC)GATCCAAAACTCTCACACAGACATAAACGCA NGG 10 8 17 8 24 0 100 5 10