Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604202351_77k9 GTTGTTTGGTTTGTTTATTTCTGTGGTTCTGG(+ACTCAC)GATCCAGCCCAGGGCCTTACATGTGCTAGAC NGG 10 8 17 8 24 0 100 5 10
    240604202349_i16v TATTTGTCTAGCACATGTAAGGCCCTGGGCTG(+ACTCAC)GATCCCAGAACCACAGAAATAAACAAACCAA NGG 10 8 17 8 24 0 100 5 10
    240604202347_df6x GTTACAGCAGGGATTTGACAAAACACAGGGTG(+ACTCAC)GATCCATGCAGTTTGGAAACTTAACTTTGAA NGG 10 8 17 8 24 0 100 5 10
    240604202346_r98u GATCGTTCAAAGTTAAGTTTCCAAACTGCATG(+ACTCAC)GATCCACCCTGTGTTTTGTCAAATCCCTGCT NGG 10 8 17 8 24 0 100 5 10
    240604202344_uoda TTTCTAAGCCACACAAGATGGGGTAAAAAGGG(+ACTCAC)GTACCAACACCTCAATGGCAGTTAGACTTCC NGG 10 8 17 8 24 0 100 5 10
    240604202343_8pnn TGAGTGGAAGTCTAACTGCCATTGAGGTGTTG(+ACTCAC)GTACCCCTTTTTACCCCATCTTGTGTGGCTT NGG 10 8 17 8 24 0 100 5 10
    240604202341_od7x ATGTGAATCCAGAGCTTGCTGGGGAGCCAGAG(+ACTCAC)GTACCATTCAATGGCAGAGCTCTTCCCTAGC NGG 10 8 17 8 24 0 100 5 10
    240604202340_i8tb CTTATGCTAGGGAAGAGCTCTGCCATTGAATG(+ACTCAC)GTACCTCTGGCTCCCCAGCAAGCTCTGGATT NGG 10 8 17 8 24 0 100 5 10
    240604202339_32l5 TCCGATGTCTGATGTGTCATCTTTTTATCTTG(+ACTCAC)GATCCTTCCCTGGTTTGAAACTTTAAACTCT NGG 10 8 17 8 24 0 100 5 10
    240604202336_tfqy TTTCAAGAGTTTAAAGTTTCAAACCAGGGAAG(+ACTCAC)GATCCAAGATAAAAAGATGACACATCAGACA NGG 10 8 17 8 24 0 100 5 10