Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604202251_gvzj CGACTCAAAGGGCCTTCTTGCTGGGGCCTCTG(+ACTCAC)GATCCTCTGTCCTGGGGCCGGAAGGCAGGGG NGG 10 8 17 8 24 0 100 5 10
    240604202249_p263 TCCGGCCCCTGCCTTCCGGCCCCAGGACAGAG(+ACTCAC)GATCCAGAGGCCCCAGCAAGAAGGCCCTTTG NGG 10 8 17 8 24 0 100 5 10
    240604202247_dea7 TTGTCATTCATCACGAAGTAGGCCAAATCATG(+ACTCAC)GTACCACAGGAATTTAGAAATCTAACTGCAA NGG 10 8 17 8 24 0 100 5 10
    240604202245_2mgb CCAGATTGCAGTTAGATTTCTAAATTCCTGTG(+ACTCAC)GTACCATGATTTGGCCTACTTCGTGATGAAT NGG 10 8 17 8 24 0 100 5 10
    240604202244_w5t6 AAAGCCCTGGGTTGACTGACTCCCTCACTCAG(+ACTCAC)GATCCTAAGAACAATGCTGACTGCAGTTTTC NGG 10 8 17 8 24 0 100 5 10
    240604202243_kbk3 AAGTAGAAAACTGCAGTCAGCATTGTTCTTAG(+ACTCAC)GATCCTGAGTGAGGGAGTCAGTCAACCCAGG NGG 10 8 17 8 24 0 100 5 10
    240604202241_3z23 GTGCAAGCGCCATGGCACCCTGCTGTACCACG(+ACTCAC)GTACCAAACTCAACTGTACAGTCAACAGTCA NGG 10 8 17 8 24 0 100 5 10
    240604202239_xuhy CTCGCTGACTGTTGACTGTACAGTTGAGTTTG(+ACTCAC)GTACCGTGGTACAGCAGGGTGCCATGGCGCT NGG 10 8 17 8 24 0 100 5 10
    240604202238_pfae CGTAGGCCGATGAGTGTAAGTAAAAGAGTGTG(+ACTCAC)GTACCACTGGAAAAAACTCCAAACCAGGGCC NGG 10 8 17 8 24 0 100 5 10
    240604202236_p3q0 TCGATGGCCCTGGTTTGGAGTTTTTTCCAGTG(+ACTCAC)GTACCACACTCTTTTACTTACACTCATCGGC NGG 10 8 17 8 24 0 100 5 10