Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604202201_nn5o AGCTTCCTCTCTGTAGTTCCCTGAGGTGGGAG(+ACTCAC)GTACCCACTTTGCCATGCATAACTCATCTCT NGG 10 8 17 8 24 0 100 5 10
    240604202159_mqhf GGAAGAGAGATGAGTTATGCATGGCAAAGTGG(+ACTCAC)GTACCTCCCACCTCAGGGAACTACAGAGAGG NGG 10 8 17 8 24 0 100 5 10
    240604202158_jgi9 CCTGTGCCCACTGGAGCCAGAAGAGGGCATTG(+ACTCAC)GATCCTGAAACTGAAGTTACAGATGTTGGGA NGG 10 8 17 8 24 0 100 5 10
    240604202157_0g7l GTGGCTCCCAACATCTGTAACTTCAGTTTCAG(+ACTCAC)GATCCAATGCCCTCTTCTGGCTCCAGTGGGC NGG 10 8 17 8 24 0 100 5 10
    240604202155_xp9w AGGGCTGAGAAGGTAGGAGCAGTACTGCAGGG(+ACTCAC)GATCCTAAGCCAGAGGTCTGGTTAATTTACA NGG 10 8 17 8 24 0 100 5 10
    240604202154_xi5s TATGGTGTAAATTAACCAGACCTCTGGCTTAG(+ACTCAC)GATCCCCTGCAGTACTGCTCCTACCTTCTCA NGG 10 8 17 8 24 0 100 5 10
    240604202153_xz93 TCCTCTGAACTCTCTCATCTACCTGAAAACTG(+ACTCAC)GATCCACAGAACAGTTAACTTTGATCCCACC NGG 10 8 17 8 24 0 100 5 10
    240604202151_t1qm CTCAGGGTGGGATCAAAGTTAACTGTTCTGTG(+ACTCAC)GATCCAGTTTTCAGGTAGATGAGAGAGTTCA NGG 10 8 17 8 24 0 100 5 10
    240604202150_keof ACCAGTGTTCTTTATACACTCTATTCCAGGTG(+ACTCAC)GATCCTTTGACATTTACGTGGTTTGGTTGGT NGG 10 8 17 8 24 0 100 5 10
    240604202149_lmbu ATGCCACCAACCAAACCACGTAAATGTCAAAG(+ACTCAC)GATCCACCTGGAATAGAGTGTATAAAGAACA NGG 10 8 17 8 24 0 100 5 10