Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604202056_1bmu GATAGACATTATCTCCCCCCCCCCTTTTTCAG(+ACTCAC)GTACCTGTAATTTGTGTCTGAGAGGCAGGGT NGG 10 8 17 8 24 0 100 5 10
    240604202055_uh9g TTCCCACCCTGCCTCTCAGACACAAATTACAG(+ACTCAC)GTACCTGAAAAAGGGGGGGGGGAGATAATGT NGG 10 8 17 8 24 0 100 5 10
    240604202054_l6n9 GGTGTCATCGTTTGGAGGCTGATTATGGGATG(+ACTCAC)GATCCCCGGGTATGGCAGTCTCAGAATACTT NGG 10 8 17 8 24 0 100 5 10
    240604202051_sj7p GATAAAAGTATTCTGAGACTGCCATACCCGGG(+ACTCAC)GATCCATCCCATAATCAGCCTCCAAACGATG NGG 10 8 17 8 24 0 100 5 10
    240604202049_gduc AGGTATTCCCCGGTCCCTACAGCTAATCCATG(+ACTCAC)GATCCCTGAAAAGGGACTGCAGGCAGGACAG NGG 10 8 17 8 24 0 100 5 10
    240604202047_7hfk CAGTACTGTCCTGCCTGCAGTCCCTTTTCAGG(+ACTCAC)GATCCATGGATTAGCTGTAGGGACCGGGGAA NGG 10 8 17 8 24 0 100 5 10
    240604202045_45ul TTTCATAACTTCAATAGCTTTTTTCTTTTCGG(+ACTCAC)GATCCCAGTGTTGGCTCTATTTCACTTTCTA NGG 10 8 17 8 24 0 100 5 10
    240604202044_2fbr ATTATTAGAAAGTGAAATAGAGCCAACACTGG(+ACTCAC)GATCCCGAAAAGAAAAAAGCTATTGAAGTTA NGG 10 8 17 8 24 0 100 5 10
    240604202043_27c3 GTTTTCTCCTCCAACGAAAACCTCAAGTTCAG(+ACTCAC)GTACCACTGAAAAGAGACTAGTTTGAAAAGA NGG 10 8 17 8 24 0 100 5 10
    240604202041_i4or TAGTCTCTTTTCAAACTAGTCTCTTTTCAGTG(+ACTCAC)GTACCTGAACTTGAGGTTTTCGTTGGAGGAG NGG 10 8 17 8 24 0 100 5 10